Expression of O6-alkylguanine-DNA alkyltransferase in normal and malignant bladder tissue of Egyptian patients

Abir A. Saad; Heba Sh Kassem; Andrew C. Povey; Geoffrey P. Margison

doi:10.4061/2010/840230

Expression of O6-alkylguanine-DNA alkyltransferase in normal and malignant bladder tissue of Egyptian patients

Abir A. Saad, Heba Sh Kassem, Andrew C. Povey, Geoffrey P. Margison

Research output: Contribution to journal › Article › peer-review

Abstract

Bladder tumour tissues and corresponding uninvolved mucosa (normal tissue) of Egyptian bladder cancer patients were assessed for O6 -alkylguanine-DNA-alkyltransferase (MGMT) activity by functional assay of tissue extracts (36 paired samples), and distribution by immunofluorescence (IF) microscopy of fixed material (24 paired samples). MGMT varied widely from 42-253 fmoles/mg protein and from 3.2-40 fmoles/μ g DNA in normal and 58-468 fmoles/mg protein and 2.5-49.5 fmoles/mg protein, in the tumour tissues; only one tumour had undetectable activity. Pairwise comparison of MGMT activity in tumour and adjacent normal tissue showed no significant difference based on DNA content but was 1.75-fold higher in tumour (P

Original language	English
Article number	840230
Journal	Journal of Nucleic Acids
Volume	2010
DOIs	https://doi.org/10.4061/2010/840230
Publication status	Published - 2010

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title = "Expression of O6-alkylguanine-DNA alkyltransferase in normal and malignant bladder tissue of Egyptian patients",

abstract = "Bladder tumour tissues and corresponding uninvolved mucosa (normal tissue) of Egyptian bladder cancer patients were assessed for O6 -alkylguanine-DNA-alkyltransferase (MGMT) activity by functional assay of tissue extracts (36 paired samples), and distribution by immunofluorescence (IF) microscopy of fixed material (24 paired samples). MGMT varied widely from 42-253 fmoles/mg protein and from 3.2-40 fmoles/μ g DNA in normal and 58-468 fmoles/mg protein and 2.5-49.5 fmoles/mg protein, in the tumour tissues; only one tumour had undetectable activity. Pairwise comparison of MGMT activity in tumour and adjacent normal tissue showed no significant difference based on DNA content but was 1.75-fold higher in tumour (P",

author = "Saad, {Abir A.} and Kassem, {Heba Sh} and Povey, {Andrew C.} and Margison, {Geoffrey P.}",

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doi = "10.4061/2010/840230",

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T1 - Expression of O6-alkylguanine-DNA alkyltransferase in normal and malignant bladder tissue of Egyptian patients

AU - Saad, Abir A.

AU - Kassem, Heba Sh

AU - Povey, Andrew C.

AU - Margison, Geoffrey P.

PY - 2010

Y1 - 2010

N2 - Bladder tumour tissues and corresponding uninvolved mucosa (normal tissue) of Egyptian bladder cancer patients were assessed for O6 -alkylguanine-DNA-alkyltransferase (MGMT) activity by functional assay of tissue extracts (36 paired samples), and distribution by immunofluorescence (IF) microscopy of fixed material (24 paired samples). MGMT varied widely from 42-253 fmoles/mg protein and from 3.2-40 fmoles/μ g DNA in normal and 58-468 fmoles/mg protein and 2.5-49.5 fmoles/mg protein, in the tumour tissues; only one tumour had undetectable activity. Pairwise comparison of MGMT activity in tumour and adjacent normal tissue showed no significant difference based on DNA content but was 1.75-fold higher in tumour (P

AB - Bladder tumour tissues and corresponding uninvolved mucosa (normal tissue) of Egyptian bladder cancer patients were assessed for O6 -alkylguanine-DNA-alkyltransferase (MGMT) activity by functional assay of tissue extracts (36 paired samples), and distribution by immunofluorescence (IF) microscopy of fixed material (24 paired samples). MGMT varied widely from 42-253 fmoles/mg protein and from 3.2-40 fmoles/μ g DNA in normal and 58-468 fmoles/mg protein and 2.5-49.5 fmoles/mg protein, in the tumour tissues; only one tumour had undetectable activity. Pairwise comparison of MGMT activity in tumour and adjacent normal tissue showed no significant difference based on DNA content but was 1.75-fold higher in tumour (P

U2 - 10.4061/2010/840230

DO - 10.4061/2010/840230

M3 - Article

SN - 2090-0201

VL - 2010

JO - Journal of Nucleic Acids

JF - Journal of Nucleic Acids

M1 - 840230

ER -

Expression of O6-alkylguanine-DNA alkyltransferase in normal and malignant bladder tissue of Egyptian patients

Abstract

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