Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes

Jeffrey I. Penny; Simone T. Hall; Charles J. Woodrow; Gill M. Cowan; Annette M. Gero; Sanjeev Krishna

doi:10.1016/S0166-6851(98)00024-3

Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes

Jeffrey I. Penny, Simone T. Hall, Charles J. Woodrow, Gill M. Cowan, Annette M. Gero, Sanjeev Krishna

Research output: Contribution to journal › Article › peer-review

Abstract

When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, that several specific nutrient transporters are the product of P. falciparum genes. We report the expression of nucleoside, nucleobase, hexose and monocarboxylate transport systems in Xenopus oocytes when injected with mRNA isolated from asexual stages of developing P. falciparum parasites. Their properties are distinct from transport events occurring at the infected erythrocyte membrane or the electrophysiologically identified channel localised to the parasitophorous vacuolar membrane. These novel transporters are substrate-specific and stereoselective, and represent a key regulatory step in the acquisition and export of metabolites by intraerythrocytic P. falciparum. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	81-89
Number of pages	8
Journal	Molecular and biochemical parasitology
Volume	93
Issue number	1
DOIs	https://doi.org/10.1016/S0166-6851(98)00024-3
Publication status	Published - 15 May 1998

Keywords

Heterologous expression
Malaria
Transport
Xenopus oocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0166-6851(98)00024-3

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title = "Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes",

abstract = "When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, that several specific nutrient transporters are the product of P. falciparum genes. We report the expression of nucleoside, nucleobase, hexose and monocarboxylate transport systems in Xenopus oocytes when injected with mRNA isolated from asexual stages of developing P. falciparum parasites. Their properties are distinct from transport events occurring at the infected erythrocyte membrane or the electrophysiologically identified channel localised to the parasitophorous vacuolar membrane. These novel transporters are substrate-specific and stereoselective, and represent a key regulatory step in the acquisition and export of metabolites by intraerythrocytic P. falciparum. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "Heterologous expression, Malaria, Transport, Xenopus oocytes",

author = "Penny, {Jeffrey I.} and Hall, {Simone T.} and Woodrow, {Charles J.} and Cowan, {Gill M.} and Gero, {Annette M.} and Sanjeev Krishna",

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T1 - Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes

AU - Penny, Jeffrey I.

AU - Hall, Simone T.

AU - Woodrow, Charles J.

AU - Cowan, Gill M.

AU - Gero, Annette M.

AU - Krishna, Sanjeev

PY - 1998/5/15

Y1 - 1998/5/15

N2 - When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, that several specific nutrient transporters are the product of P. falciparum genes. We report the expression of nucleoside, nucleobase, hexose and monocarboxylate transport systems in Xenopus oocytes when injected with mRNA isolated from asexual stages of developing P. falciparum parasites. Their properties are distinct from transport events occurring at the infected erythrocyte membrane or the electrophysiologically identified channel localised to the parasitophorous vacuolar membrane. These novel transporters are substrate-specific and stereoselective, and represent a key regulatory step in the acquisition and export of metabolites by intraerythrocytic P. falciparum. Copyright (C) 1998 Elsevier Science B.V.

AB - When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, that several specific nutrient transporters are the product of P. falciparum genes. We report the expression of nucleoside, nucleobase, hexose and monocarboxylate transport systems in Xenopus oocytes when injected with mRNA isolated from asexual stages of developing P. falciparum parasites. Their properties are distinct from transport events occurring at the infected erythrocyte membrane or the electrophysiologically identified channel localised to the parasitophorous vacuolar membrane. These novel transporters are substrate-specific and stereoselective, and represent a key regulatory step in the acquisition and export of metabolites by intraerythrocytic P. falciparum. Copyright (C) 1998 Elsevier Science B.V.

KW - Heterologous expression

KW - Malaria

KW - Transport

KW - Xenopus oocytes

U2 - 10.1016/S0166-6851(98)00024-3

DO - 10.1016/S0166-6851(98)00024-3

M3 - Article

SN - 0166-6851

VL - 93

SP - 81

EP - 89

JO - Molecular and biochemical parasitology

JF - Molecular and biochemical parasitology

IS - 1

ER -

Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes

Abstract

Keywords

UN SDGs

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