Expression pattern and cellular distribution of the murine homologue of AF10

Britta Linder, Louise K. Jones, Tracy Chaplin, Adone Mohd-Sarip, Uwe A O Heinlein, Bryan D. Young, Vaskar Saha

    Research output: Contribution to journalArticlepeer-review


    We have cloned Af10, the murine homologue of the MLL partner gene AF10. The predicted open reading frame of Af10 contains 1069 aa which are 90% identical to those of AF10. Af10 contains an N-terminal cysteine-rich region with a LAP/PHD finger, a leucine zipper domain and a glutamine-rich region at the C-terminus, features also found in the human proteins AF10 and AF17. A single 5.5-kb transcript was detected in murine tissues with the highest level of expression in the testes. A polyclonal antibody raised to the cysteine-rich region of AF10 was able to identify a double band of 140 kDa on Western analysis in mouse testicular extracts. After subcellular separation Af10 was identified in both the nuclear and cytoplasmic extracts, again as a double band of 140 kDa in size. In situ hybridisation studies were performed with sense and antisense digoxigenin-labelled oligonucleotides. High levels of expression were noted in postmeiotic germ cells, especially in spermatids from around stage VI to stage VIII. High levels of expression were also seen in the white matter of the cerebellum, extending into the granular layer. The expression in differentiated rather than in proliferating cells suggests that the role of Af10 may lie in the suppression of proliferation rather than in differentiation. Since the LAP/PHD finger domains are lost in the MLL-AF10 fusion, arguably such a function could be carried out by this domain.
    Original languageEnglish
    Pages (from-to)285-296
    Number of pages11
    JournalBBA - Gene Structure and Expression
    Issue number3
    Publication statusPublished - 22 Dec 1998


    • AF10
    • Cellular distribution
    • Expression pattern
    • Murine homologue


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