Abstract
BACKGROUND:
This work focuses on combining electrospun biodegradable poly-DL-lactide (PDLLA) fibres and 45S5 Bioglass® for tissue engineering applications.
RESULTS:
A variety of fibrous structures were produced upon application of an electric field to a flowing solution of PDLLA (5 wt/v%) in di-methyl carbonate (DMC). Electrospinning was achieved at an applied voltage of 8.5 kV for a fixed flow rate of 5 μ Lmin-1. Scanning electron microscopy images of PDLLA fibres deposited on 45S5 Bioglass® sintered pellets revealed that the fibres had diameters in the range 100-200 nm, leading to increased surface roughness, as assessed by white light interferometry. Bioactivity studies on PDLLA fibre coated Bioglass® substrates were carried out in simulated body fluid (SBF) for 7, 14 and 28 days. It was found that mineralization of PDLLA fibres on 45S5 Bioglass® substrate (formation of hydroxyapatite) occurred after 7 days of immersion in SBF and full coverage of PDLLA fibres with HA nanocrystals was achieved after 14 days in SBF. CONCLUSION:
The approach investigated represents a convenient method to develop a controlled mineralized fibrous topography on bioactive glass substrates for improved cell attachment, which can be exploited in bone tissue engineering applications.
This work focuses on combining electrospun biodegradable poly-DL-lactide (PDLLA) fibres and 45S5 Bioglass® for tissue engineering applications.
RESULTS:
A variety of fibrous structures were produced upon application of an electric field to a flowing solution of PDLLA (5 wt/v%) in di-methyl carbonate (DMC). Electrospinning was achieved at an applied voltage of 8.5 kV for a fixed flow rate of 5 μ Lmin-1. Scanning electron microscopy images of PDLLA fibres deposited on 45S5 Bioglass® sintered pellets revealed that the fibres had diameters in the range 100-200 nm, leading to increased surface roughness, as assessed by white light interferometry. Bioactivity studies on PDLLA fibre coated Bioglass® substrates were carried out in simulated body fluid (SBF) for 7, 14 and 28 days. It was found that mineralization of PDLLA fibres on 45S5 Bioglass® substrate (formation of hydroxyapatite) occurred after 7 days of immersion in SBF and full coverage of PDLLA fibres with HA nanocrystals was achieved after 14 days in SBF. CONCLUSION:
The approach investigated represents a convenient method to develop a controlled mineralized fibrous topography on bioactive glass substrates for improved cell attachment, which can be exploited in bone tissue engineering applications.
| Original language | English |
|---|---|
| Pages (from-to) | 768-774 |
| Number of pages | 7 |
| Journal | Journal of Chemical Technology and Biotechnology |
| Volume | 85 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 27 Oct 2009 |
Keywords
- Bioactive glass substrates
- Bioactivity studies
- Electrospinning
- Light interferometry
- PDLLA fibres
- SBF
- Tissue engineering