Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy

T. J. Harvey, E. Gazi, A. Henderson, R. D. Snook, N. W. Clarke, M. Brown, P. Gardner

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    Abstract

    In this study we obtained Fourier transform infrared (FTIR) spectra of fixed prostate cell lines of differing types as well as the primary epithelial cells from benign prostatic hyperplasia (BPH). Results showed that by using multivariate chemometric analysis it was possible to discriminate and classify these cell lines, which gave rise to sensitivity and specificity values of >94% and >98%, respectively. Following on from these results the possible influences of different factors on the discrimination and classification of the prostate cell lines were examined. Firstly, the effect of using different growth media during cell culturing was investigated, with results indicating that this did not influence chemometric discrimination. Secondly, differences in the nucleus-to-cytoplasm (N/C) ratio were examined, and it was concluded that this factor was not the main reason for the discrimination and classification of the prostate cancer (CaP) cell lines. In conclusion, given the fact that neither growth media nor N/C ratio could totally explain the classification it is likely that actual biochemical differences between the cell lines is the major contributing factor. © 2009 Royal Society of Chemistry.
    Original languageEnglish
    Pages (from-to)1083-1091
    Number of pages8
    JournalAnalyst
    Volume134
    Issue number6
    DOIs
    Publication statusPublished - 2009

    Keywords

    • infrared micro-spectroscopy
    • ir microspectroscopy
    • chemometric analysis
    • raman-spectroscopy
    • diagnosis
    • identification
    • carcinoma
    • cytology
    • spectra
    • adenocarcinoma

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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