Fas mRNA expression in blood is reduced during episodes of human corneal graft rejection

Mary Hillarby; Janet A. Dixon; Irena Reynolds; Eleftheria Zeggini; Andrew B. Tullo; M. Chantal Hillarby

Fas mRNA expression in blood is reduced during episodes of human corneal graft rejection

Mary Hillarby, Janet A. Dixon, Irena Reynolds, Eleftheria Zeggini, Andrew B. Tullo, M. Chantal Hillarby

Research output: Contribution to journal › Article › peer-review

Abstract

Background. Our purpose is to examine levels of Fas mRNA expression in blood during human corneal transplant rejection. Methods. Fas mRNA expression was detected by reverse transcription-PCR in blood from normal controls, corneal recipients at the time of transplantation and during episodes of rejection. Results. Samples taken at the time of a corneal rejection episode showed Fas mRNA levels were significantly lower in these patients than either normal controls (P=0.017) or corneal transplant recipients not undergoing graft rejection (P=0.00052). Serial samples from five patients who suffered an episode of rejection showed that the level of Fas mRNA is reduced during the rejection episode and subsequently recovers. Conclusions. These results indicate low levels of Fas mRNA in blood may have a role in corneal transplant rejection.

Original language	English
Pages (from-to)	702-705
Number of pages	3
Journal	Transplantation
Volume	71
Issue number	5
Publication status	Published - 15 Mar 2001

Keywords

Adult
genetics: Antigens, CD95
immunology: Corneal Transplantation
blood: Graft Rejection
Humans
Middle Aged
blood: RNA, Messenger
Research Support, Non-U.S. Gov't

Cite this

@article{0480059eab044438bac13e77afe438f1,

title = "Fas mRNA expression in blood is reduced during episodes of human corneal graft rejection",

abstract = "Background. Our purpose is to examine levels of Fas mRNA expression in blood during human corneal transplant rejection. Methods. Fas mRNA expression was detected by reverse transcription-PCR in blood from normal controls, corneal recipients at the time of transplantation and during episodes of rejection. Results. Samples taken at the time of a corneal rejection episode showed Fas mRNA levels were significantly lower in these patients than either normal controls (P=0.017) or corneal transplant recipients not undergoing graft rejection (P=0.00052). Serial samples from five patients who suffered an episode of rejection showed that the level of Fas mRNA is reduced during the rejection episode and subsequently recovers. Conclusions. These results indicate low levels of Fas mRNA in blood may have a role in corneal transplant rejection.",

keywords = "Adult, genetics: Antigens, CD95, immunology: Corneal Transplantation, blood: Graft Rejection, Humans, Middle Aged, blood: RNA, Messenger, Research Support, Non-U.S. Gov't",

author = "Mary Hillarby and Dixon, {Janet A.} and Irena Reynolds and Eleftheria Zeggini and Tullo, {Andrew B.} and {Chantal Hillarby}, M.",

year = "2001",

month = mar,

day = "15",

language = "English",

volume = "71",

pages = "702--705",

journal = "Transplantation",

issn = "1534-6080",

publisher = "Lippincott Williams & Wilkins",

number = "5",

}

TY - JOUR

T1 - Fas mRNA expression in blood is reduced during episodes of human corneal graft rejection

AU - Hillarby, Mary

AU - Dixon, Janet A.

AU - Reynolds, Irena

AU - Zeggini, Eleftheria

AU - Tullo, Andrew B.

AU - Chantal Hillarby, M.

PY - 2001/3/15

Y1 - 2001/3/15

N2 - Background. Our purpose is to examine levels of Fas mRNA expression in blood during human corneal transplant rejection. Methods. Fas mRNA expression was detected by reverse transcription-PCR in blood from normal controls, corneal recipients at the time of transplantation and during episodes of rejection. Results. Samples taken at the time of a corneal rejection episode showed Fas mRNA levels were significantly lower in these patients than either normal controls (P=0.017) or corneal transplant recipients not undergoing graft rejection (P=0.00052). Serial samples from five patients who suffered an episode of rejection showed that the level of Fas mRNA is reduced during the rejection episode and subsequently recovers. Conclusions. These results indicate low levels of Fas mRNA in blood may have a role in corneal transplant rejection.

AB - Background. Our purpose is to examine levels of Fas mRNA expression in blood during human corneal transplant rejection. Methods. Fas mRNA expression was detected by reverse transcription-PCR in blood from normal controls, corneal recipients at the time of transplantation and during episodes of rejection. Results. Samples taken at the time of a corneal rejection episode showed Fas mRNA levels were significantly lower in these patients than either normal controls (P=0.017) or corneal transplant recipients not undergoing graft rejection (P=0.00052). Serial samples from five patients who suffered an episode of rejection showed that the level of Fas mRNA is reduced during the rejection episode and subsequently recovers. Conclusions. These results indicate low levels of Fas mRNA in blood may have a role in corneal transplant rejection.

KW - Adult

KW - genetics: Antigens, CD95

KW - immunology: Corneal Transplantation

KW - blood: Graft Rejection

KW - Humans

KW - Middle Aged

KW - blood: RNA, Messenger

KW - Research Support, Non-U.S. Gov't

M3 - Article

SN - 1534-6080

VL - 71

SP - 702

EP - 705

JO - Transplantation

JF - Transplantation

IS - 5

ER -

Fas mRNA expression in blood is reduced during episodes of human corneal graft rejection

Abstract

Keywords

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