FLT3-ITDs Instruct a Myeloid Differentiation and Transformation Bias in Lymphomyeloid Multipotent Progenitors

AdamJ Mead, Shabnam Kharazi, Deborah Atkinson, Iain Macaulay, Christian Pecquet, Stephen Loughran, Michael Lutteropp, Petter Woll, Onima Chowdhury, Sidinh Luc, Natalija Buza-Vidas, Helen Ferry, Sally Ann Clark, Nicolas Goardon, Paresh Vyas, StefanN Constantinescu, Ewa Sitnicka, Claus Nerlov, StenEirikW Jacobsen

    Research output: Contribution to journalArticlepeer-review


    Whether signals mediated via growth factor receptors (GFRs) might influence lineage fate in multipotent progenitors (MPPs) is unclear. We explored this issue in a mouse knockin model of gain-of-function Flt3-ITD mutation because FLT3-ITDs are paradoxically restricted to acute myeloid leukemia even though Flt3 primarily promotes lymphoid development during normal hematopoiesis. When expressed in MPPs, Flt3-ITD collaborated with Runx1 mutation to induce high-penetrance aggressive leukemias that were exclusively of the myeloid phenotype. Flt3-ITDs preferentially expanded MPPs with reduced lymphoid and increased myeloid transcriptional priming while compromising early B and T lymphopoiesis. Flt3-ITD-induced myeloid lineage bias involved upregulation of the transcription factor Pu.1, which is a direct target gene of Stat3, an aberrantly activated target of Flt3-ITDs, further establishing how lineage bias can be inflicted on MPPs through aberrant GFR signaling. Collectively, these findings provide new insights into how oncogenic mutations might subvert the normal process of lineage commitment and dictate the phenotype of resulting malignancies. © 2013 The Authors.
    Original languageEnglish
    Pages (from-to)1766-1776
    Number of pages10
    JournalCell Reports
    Issue number6
    Publication statusPublished - 27 Mar 2013

    Research Beacons, Institutes and Platforms

    • Manchester Cancer Research Centre


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