Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

Wei-Tao Dou; Li-Fang Liu; Jie Gao; Yi Zang; Guo-Rong Chen; Robert A. Field; Tony D. James; Jia Li; Xiao-Peng He

doi:10.1039/C9CC06399D

Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

Wei-Tao Dou, Li-Fang Liu, Jie Gao, Yi Zang, Guo-Rong Chen, Robert A. Field, Tony D. James, Jia Li, Xiao-Peng He

Research output: Contribution to journal › Article › peer-review

Abstract

Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.

Original language	English
Journal	Chemical Communications
DOIs	https://doi.org/10.1039/C9CC06399D
Publication status	Published - 2 Oct 2019

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Manchester Institute of Biotechnology

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10.1039/C9CC06399D

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title = "Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material",

abstract = "Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.",

author = "Wei-Tao Dou and Li-Fang Liu and Jie Gao and Yi Zang and Guo-Rong Chen and Field, {Robert A.} and James, {Tony D.} and Jia Li and Xiao-Peng He",

year = "2019",

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doi = "10.1039/C9CC06399D",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

AU - Dou, Wei-Tao

AU - Liu, Li-Fang

AU - Gao, Jie

AU - Zang, Yi

AU - Chen, Guo-Rong

AU - Field, Robert A.

AU - James, Tony D.

AU - Li, Jia

AU - He, Xiao-Peng

PY - 2019/10/2

Y1 - 2019/10/2

N2 - Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.

AB - Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.

U2 - 10.1039/C9CC06399D

DO - 10.1039/C9CC06399D

M3 - Article

SN - 1359-7345

JO - Chemical Communications

JF - Chemical Communications

ER -

Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

Abstract

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