Fluorescent probe for detection of bacteria: Conformational trigger upon bacterial reduction of an azo bridge

Nicholas J W Rattray; Waleed A. Zalloum; David Mansell; Joe Latimer; Carl H. Schwalbe; Alexander J. Blake; Elena V. Bichenkova; Sally Freeman

doi:10.1039/c2cc32521g

Fluorescent probe for detection of bacteria: Conformational trigger upon bacterial reduction of an azo bridge

Nicholas J W Rattray, Waleed A. Zalloum, David Mansell, Joe Latimer, Carl H. Schwalbe, Alexander J. Blake, Elena V. Bichenkova, Sally Freeman

Research output: Contribution to journal › Article › peer-review

Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer. © 2012 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	6393-6395
Number of pages	2
Journal	Chemical Communications
Volume	48
Issue number	51
DOIs	https://doi.org/10.1039/c2cc32521g
Publication status	Published - 28 Jun 2012

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10.1039/c2cc32521g

CCDC 832284: Experimental Crystal Structure Determination
Rattray, N. J. W. (Contributor), Zalloum, W. A. (Contributor), Mansell, D. (Contributor), Latimer, J. (Contributor), Schwalbe, C. H. (Contributor), Blake, A. J. (Contributor), Bichenkova, E. (Contributor) & Freeman, S. (Contributor), Cambridge Crystallographic Data Centre, 1 Jan 2012
DOI: 10.5517/ccwy1wq, http://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccwy1wq&sid=DataCite
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title = "Fluorescent probe for detection of bacteria: Conformational trigger upon bacterial reduction of an azo bridge",

abstract = "Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer. {\textcopyright} 2012 The Royal Society of Chemistry.",

author = "Rattray, {Nicholas J W} and Zalloum, {Waleed A.} and David Mansell and Joe Latimer and Schwalbe, {Carl H.} and Blake, {Alexander J.} and Bichenkova, {Elena V.} and Sally Freeman",

year = "2012",

month = jun,

day = "28",

doi = "10.1039/c2cc32521g",

language = "English",

volume = "48",

pages = "6393--6395",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "51",

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TY - JOUR

T1 - Fluorescent probe for detection of bacteria: Conformational trigger upon bacterial reduction of an azo bridge

AU - Rattray, Nicholas J W

AU - Zalloum, Waleed A.

AU - Mansell, David

AU - Latimer, Joe

AU - Schwalbe, Carl H.

AU - Blake, Alexander J.

AU - Bichenkova, Elena V.

AU - Freeman, Sally

PY - 2012/6/28

Y1 - 2012/6/28

N2 - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer. © 2012 The Royal Society of Chemistry.

AB - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer. © 2012 The Royal Society of Chemistry.

U2 - 10.1039/c2cc32521g

DO - 10.1039/c2cc32521g

M3 - Article

C2 - 22618019

SN - 1359-7345

VL - 48

SP - 6393

EP - 6395

JO - Chemical Communications

JF - Chemical Communications

IS - 51

ER -

Fluorescent probe for detection of bacteria: Conformational trigger upon bacterial reduction of an azo bridge

Abstract

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CCDC 832284: Experimental Crystal Structure Determination

Cite this