FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells

S. Bolte; C. Talbot; Y. Boutte; O. Catrice; N. D. Read; B. Satiat-Jeunemaitre

doi:10.1111/j.0022-2720.2004.01348.x

FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells

S. Bolte, C. Talbot, Y. Boutte, O. Catrice, N. D. Read, B. Satiat-Jeunemaitre

Research output: Contribution to journal › Article › peer-review

Abstract

FM-dyes are widely used to study endocytosis, vesicle trafficking and organelle organization in living eukaryotic cells. The increasing use of FM-dyes in plant cells has provoked much debate with regard to their suitability as endocytosis markers, which organelles they stain and the precise pathways they follow through the vesicle trafficking network. A primary aim of this article is to assess critically the current status of this debate in plant cells. For this purpose, background information on the important characteristics of the FM-dyes, and of optimal dye concentrations, conditions of dye storage, and staining and imaging protocols, are provided. Particular emphasis is placed on using the FM-dyes in double labelling experiments to identity specific organelles. In this way, staining of the Golgi with FM4-64 has been demonstrated for the first time.

Original language	English
Pages (from-to)	159-173
Number of pages	14
Journal	Journal of Microscopy
Volume	214
Issue number	2
DOIs	https://doi.org/10.1111/j.0022-2720.2004.01348.x
Publication status	Published - May 2004

Keywords

Confocal microscopy
Endocytosis
FM-dyes
Membrane recycling
Plant cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.0022-2720.2004.01348.x

Cite this

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title = "FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells",

abstract = "FM-dyes are widely used to study endocytosis, vesicle trafficking and organelle organization in living eukaryotic cells. The increasing use of FM-dyes in plant cells has provoked much debate with regard to their suitability as endocytosis markers, which organelles they stain and the precise pathways they follow through the vesicle trafficking network. A primary aim of this article is to assess critically the current status of this debate in plant cells. For this purpose, background information on the important characteristics of the FM-dyes, and of optimal dye concentrations, conditions of dye storage, and staining and imaging protocols, are provided. Particular emphasis is placed on using the FM-dyes in double labelling experiments to identity specific organelles. In this way, staining of the Golgi with FM4-64 has been demonstrated for the first time.",

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T1 - FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells

AU - Bolte, S.

AU - Talbot, C.

AU - Boutte, Y.

AU - Catrice, O.

AU - Read, N. D.

AU - Satiat-Jeunemaitre, B.

PY - 2004/5

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AB - FM-dyes are widely used to study endocytosis, vesicle trafficking and organelle organization in living eukaryotic cells. The increasing use of FM-dyes in plant cells has provoked much debate with regard to their suitability as endocytosis markers, which organelles they stain and the precise pathways they follow through the vesicle trafficking network. A primary aim of this article is to assess critically the current status of this debate in plant cells. For this purpose, background information on the important characteristics of the FM-dyes, and of optimal dye concentrations, conditions of dye storage, and staining and imaging protocols, are provided. Particular emphasis is placed on using the FM-dyes in double labelling experiments to identity specific organelles. In this way, staining of the Golgi with FM4-64 has been demonstrated for the first time.

KW - Confocal microscopy

KW - Endocytosis

KW - FM-dyes

KW - Membrane recycling

KW - Plant cells

U2 - 10.1111/j.0022-2720.2004.01348.x

DO - 10.1111/j.0022-2720.2004.01348.x

M3 - Article

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ER -

FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells

Abstract

Keywords

UN SDGs

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