Formation and identification of trimethylimidazole during tetramethylpyrazine production from glucose by Bacillus strains

Zijun Xiao, Jian R. Lu, Cuiqing Ma, Ping Xu

    Research output: Contribution to journalArticlepeer-review

    Abstract

    During 2,3,5,6-tetramethylpyrazine production from glucose by Bacillus strains, a novel product was detected and identified as 2,4,5-trimethylimidazole (TMI) by GC/MS. TMI appeared in the culture medium only after glucose had been depleted and then increased to 0.25-0.31 g l-1 in 90-120 h. When the ammonium source was changed from (NH4)2SO4 to (NH4)2HPO4, only about one tenth of TMI was detected. Although the mechanistic events largely remain unclear, both microbial strains tested demonstrated similar dynamic processes of TMI production, suggesting that TMI formation is a genuine feature of Bacillus species. © Springer Science+Business Media B.V. 2009.
    Original languageEnglish
    Pages (from-to)1421-1425
    Number of pages4
    JournalBiotechnology Letters
    Volume31
    Issue number9
    DOIs
    Publication statusPublished - Aug 2009

    Keywords

    • Bacillus
    • Identification
    • Metabolite
    • Trimethylimidazole

    Fingerprint

    Dive into the research topics of 'Formation and identification of trimethylimidazole during tetramethylpyrazine production from glucose by Bacillus strains'. Together they form a unique fingerprint.

    Cite this