Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

Martin J. Weissenborn; Johannes W. Wehner; Christopher J. Gray; Robert Šardzík; Claire E. Eyers; Thisbe K. Lindhorst; Sabine L. Flitsch; Robert Sardzik

doi:10.3762/bjoc.8.86

Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

Martin J. Weissenborn, Johannes W. Wehner, Christopher J. Gray, Robert Šardzík, Claire E. Eyers, Thisbe K. Lindhorst, Sabine L. Flitsch, Robert Sardzik

The University of Manchester

Research output: Contribution to journal › Article › peer-review

Abstract

Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectrometry analysis after aluminium tape was attached to the underside of the slides. Furthermore, the trityl group appeared to act as an internal matrix and no additional matrix was necessary for the MS analysis. Thus, trityl groups can be used as simple hydrophobic, noncovalently linked anchors for ligands on surfaces and at the same time facilitate the in situ mass spectrometric analysis of such ligands. © 2012 The Surface Science Society of Japan.

Original language	English
Pages (from-to)	753-762
Number of pages	9
Journal	Beilstein Journal of Organic Chemistry
Volume	8
DOIs	https://doi.org/10.3762/bjoc.8.86
Publication status	Published - 21 May 2012

Keywords

Carbohydrate array
Conductive tape
MALDI-TOF MS
Nonconductive surface
Trityl-mediated adhesion

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10.3762/bjoc.8.86

http://www.beilstein-journals.org/bjoc/content/pdf/1860-5397-8-86.pdf

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title = "Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS",

abstract = "Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectrometry analysis after aluminium tape was attached to the underside of the slides. Furthermore, the trityl group appeared to act as an internal matrix and no additional matrix was necessary for the MS analysis. Thus, trityl groups can be used as simple hydrophobic, noncovalently linked anchors for ligands on surfaces and at the same time facilitate the in situ mass spectrometric analysis of such ligands. {\textcopyright} 2012 The Surface Science Society of Japan.",

keywords = "Carbohydrate array, Conductive tape, MALDI-TOF MS, Nonconductive surface, Trityl-mediated adhesion",

author = "Weissenborn, {Martin J.} and Wehner, {Johannes W.} and Gray, {Christopher J.} and Robert {\v S}ardz{\'i}k and Eyers, {Claire E.} and Lindhorst, {Thisbe K.} and Flitsch, {Sabine L.} and Robert Sardzik",

year = "2012",

month = may,

day = "21",

doi = "10.3762/bjoc.8.86",

language = "English",

volume = "8",

pages = "753--762",

journal = "Beilstein Journal of Organic Chemistry",

issn = "1860-5397",

publisher = "Beilstein-Institut ",

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TY - JOUR

T1 - Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

AU - Weissenborn, Martin J.

AU - Wehner, Johannes W.

AU - Gray, Christopher J.

AU - Šardzík, Robert

AU - Eyers, Claire E.

AU - Lindhorst, Thisbe K.

AU - Flitsch, Sabine L.

AU - Sardzik, Robert

PY - 2012/5/21

Y1 - 2012/5/21

N2 - Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectrometry analysis after aluminium tape was attached to the underside of the slides. Furthermore, the trityl group appeared to act as an internal matrix and no additional matrix was necessary for the MS analysis. Thus, trityl groups can be used as simple hydrophobic, noncovalently linked anchors for ligands on surfaces and at the same time facilitate the in situ mass spectrometric analysis of such ligands. © 2012 The Surface Science Society of Japan.

AB - Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectrometry analysis after aluminium tape was attached to the underside of the slides. Furthermore, the trityl group appeared to act as an internal matrix and no additional matrix was necessary for the MS analysis. Thus, trityl groups can be used as simple hydrophobic, noncovalently linked anchors for ligands on surfaces and at the same time facilitate the in situ mass spectrometric analysis of such ligands. © 2012 The Surface Science Society of Japan.

KW - Carbohydrate array

KW - Conductive tape

KW - MALDI-TOF MS

KW - Nonconductive surface

KW - Trityl-mediated adhesion

U2 - 10.3762/bjoc.8.86

DO - 10.3762/bjoc.8.86

M3 - Article

SN - 1860-5397

VL - 8

SP - 753

EP - 762

JO - Beilstein Journal of Organic Chemistry

JF - Beilstein Journal of Organic Chemistry

ER -

Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

Abstract

Keywords

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