Formin proteins of the DAAM subfamily play a role during axon growth

Tamás Matusek, Rita Gombos, Anita Szécsényi, Natalia Sanchez-Soriano, Ágnes Czibula, Csilla Pataki, Anita Gedai, Andreas Prokop, István Raskó, József Mihály

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The regulation of growth cone actin dynamics is a critical aspect of axonal growth control. Among the proteins that are directly involved in the regulation of actin dynamics, actin nucleation factors play a pivotal role by promoting the formation of novel actin filaments. However, the essential nucleation factors in developing neurons have so far not been clearly identified. Here, we show expression data, and use true loss-of-function analysis and targeted expression of activated constructs to demonstrate that the Drosophila formin DAAM plays a critical role in axonal morphogenesis. In agreement with this finding, we show that dDAAM is required for filopodia formation at axonal growth cones. Our genetic interaction, immunoprecipitation and protein localization studies argue that dDAAM acts in concert with Rac GTPases, Profilin and Enabled during axonal growth regulation. We also show that mouse Daam1 rescues the CNS defects observed in dDAAM mutant flies to a high degree, and vice versa, that Drosophila DAAM induces the formation of neurite-like protrusions when expressed in mouse P19 cells, strongly suggesting that the function of DAAM in developing neurons has been conserved during evolution. Copyright © 2008 Society for Neuroscience.
    Original languageEnglish
    Pages (from-to)13310-13319
    Number of pages9
    JournalJournal of Neuroscience
    Volume28
    Issue number49
    DOIs
    Publication statusPublished - 3 Dec 2008

    Keywords

    • Axon growth
    • dDAAM
    • Ena
    • Filopodia formation
    • Formin
    • Profilin
    • Rac

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