@article{9f384f8ecbc34209b77aa1ee43d1fb82,
title = "Foxm1 regulates neural progenitor fate during spinal cord regeneration",
abstract = "Xenopus tadpoles have the ability to regenerate their tails upon amputation. Although some of the molecular and cellular mechanisms that globally regulate tail regeneration have been characterised, tissue-specific response to injury remains poorly understood. Using a combination of bulk and single cell RNA sequencing on isolated spinal cords before and after amputation, we identify a number of genes specifically expressed in the spinal cord during regeneration. We show that Foxm1, a transcription factor known to promote proliferation, is essential for spinal cord regeneration. Surprisingly, Foxm1 does not control the cell cycle length of neural progenitors but regulates their fate after division. In foxm1-/- tadpoles, we observe a reduction in the number of neurons in the regenerating spinal cord, suggesting that neuronal differentiation is necessary for the regenerative process. Altogether, our data uncover a spinal cord – specific response to injury and reveal a new role for neuronal differentiation during regeneration.",
keywords = "regeneration, spinal cord, foxm1, xenopus, differentiation, scRNAseq",
author = "Diane Pelzer and Phipps, \{Lauren S.\} and Raphael Thuret and Gallardo-Dodd, \{Carlos J.\} and Baker, \{Syed Murtuza\} and Karel Dorey",
note = "Funding Information: This work was supported by a Wellcome Trust Seed Award (ref 205894/Z/17/Z), the Manchester Regenerative Medicine Network (MaRMN) and The University of Manchester Strategic Fund (ISSF) to K.D and MRC single‐cell centre award to S.M.B. (ref MR/M008908/1). D.P. and L.S.P. are supported by BBSRC‐DTP PhD studentships. L.S.P. is supported by a Biotechnology and Biological Sciences Research Council Research Training Support Grant (ref BB/M011208/1). We thank the EMBL core facility for performing the bulk RNA‐seq experiments, the Genomics Core Facility (University of Manchester) for the scRNA‐seq, the Genome Editing Unit and Histology Core Facilities for use of genotyping and cryotomy equipment (University of Manchester) and the National Xenopus Resource (NXR, Marine Biology Laboratories, Woods Hole, USA) for their bioinformatics workshop (National Xenopus Resource RRID :S CR\_013731 ). We thank Xenbase ( www.xenbase.org ) for posting Xenopus genome assemblies and annotation of literatures to assist research in the Xenopus community. We thank Andy Rowntree and Jessica Forsyth for helping to develop macros to analyse cell positions, Nancy Papalopulu for the anti‐Sox3 and anti‐Myt1 antibodies and Enrique Amaya, Raman Das and Shane Herbert for comments on the manuscript. Funding Information: This work was supported by a Wellcome Trust Seed Award (ref 205894/Z/17/Z), the Manchester Regenerative Medicine Network (MaRMN) and The University of Manchester Strategic Fund (ISSF) to K.D and MRC single-cell centre award to S.M.B. (ref MR/M008908/1). D.P. and L.S.P. are supported by BBSRC-DTP PhD studentships. L.S.P. is supported by a Biotechnology and Biological Sciences Research Council Research Training Support Grant (ref BB/M011208/1). We thank the EMBL core facility for performing the bulk RNA-seq experiments, the Genomics Core Facility (University of Manchester) for the scRNA-seq, the Genome Editing Unit and Histology Core Facilities for use of genotyping and cryotomy equipment (University of Manchester) and the National Xenopus Resource (NXR, Marine Biology Laboratories, Woods Hole, USA) for their bioinformatics workshop (National Xenopus Resource RRID:SCR\_013731). We thank Xenbase (www.xenbase.org) for posting Xenopus genome assemblies and annotation of literatures to assist research in the Xenopus community. We thank Andy Rowntree and Jessica Forsyth for helping to develop macros to analyse cell positions, Nancy Papalopulu for the anti-Sox3 and anti-Myt1 antibodies and Enrique Amaya, Raman Das and Shane Herbert for comments on the manuscript. Publisher Copyright: {\textcopyright} 2021 The Authors. Published under the terms of the CC BY 4.0 license",
year = "2021",
month = aug,
day = "24",
doi = "10.15252/embr.202050932",
language = "English",
volume = "22",
journal = "EMBO reports",
issn = "1469-221X",
publisher = "Springer Nature",
number = "9",
}