Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Eyad Elkord

doi:10.1016/j.jim.2009.06.001

Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Eyad Elkord

Research output: Contribution to journal › Article › peer-review

Abstract

Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. © 2009 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	87-90
Number of pages	3
Journal	Journal of immunological methods
Volume	347
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2009.06.001
Publication status	Published - 15 Aug 2009

Keywords

Cryopreservation
T regulatory cell

Access to Document

10.1016/j.jim.2009.06.001

Cite this

@article{3d3593ac136c495390040d28b9ae1989,

title = "Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation",

abstract = "Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. {\textcopyright} 2009 Elsevier B.V. All rights reserved.",

keywords = "Cryopreservation, T regulatory cell",

author = "Eyad Elkord",

year = "2009",

month = aug,

day = "15",

doi = "10.1016/j.jim.2009.06.001",

language = "English",

volume = "347",

pages = "87--90",

journal = "Journal of immunological methods",

issn = "0022-1759",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

AU - Elkord, Eyad

PY - 2009/8/15

Y1 - 2009/8/15

N2 - Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. © 2009 Elsevier B.V. All rights reserved.

AB - Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. © 2009 Elsevier B.V. All rights reserved.

KW - Cryopreservation

KW - T regulatory cell

U2 - 10.1016/j.jim.2009.06.001

DO - 10.1016/j.jim.2009.06.001

M3 - Article

SN - 0022-1759

VL - 347

SP - 87

EP - 90

JO - Journal of immunological methods

JF - Journal of immunological methods

IS - 1-2

ER -

Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Abstract

Keywords

Access to Document

Fingerprint

Cite this