From meiosis to mitosis - the sperm centrosome defines the kinetics of spindle assembly after fertilization in Xenopus

Tommaso Cavazza, Isabel Peset Martin, Isabelle Vernos

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Bipolar spindle assembly in the vertebrate oocyte relies on a self-organization chromosome-dependent pathway. Upon fertilization, the male gamete provides a centrosome, and the first and subsequent embryonic divisions occur in the presence of duplicated centrosomes that act as dominant microtubule organizing centres (MTOCs). The transition from meiosis to embryonic mitosis involves a necessary adaptation to integrate the dominant chromosome-dependent pathway with the centrosomes to form the bipolar spindle. Here, we took advantage of the Xenopus laevis egg extract system to mimic in vitro the assembly of the first embryonic spindle and investigate the respective contributions of the centrosome and the chromosome-dependent pathway to the kinetics of the spindle bipolarization. We found that centrosomes control the transition from the meiotic to the mitotic spindle assembly mechanism. By defining the kinetics of spindle bipolarization, the centrosomes ensure their own positioning to each spindle pole and thereby their essential correct inheritance to the two first daughter cells of the embryo for the development of a healthy organism.

    Original languageEnglish
    Pages (from-to)2538-47
    Number of pages10
    JournalJournal of Cell Science
    Volume129
    Issue number13
    DOIs
    Publication statusPublished - 1 Jul 2016

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