From mesenchymal niches to engineered in vitro model systems: Exploring and exploiting biomechanical regulation of vertebrate hedgehog signalling

Fatmah I. Ghuloum, Colin A. Johnson, Natalia A. Riobo-Del Galdo, Mahetab H. Amer

Research output: Contribution to journalArticlepeer-review

Abstract

Tissue patterning is the result of complex interactions between transcriptional programs and various mechanical cues that modulate cell behaviour and drive morphogenesis. Vertebrate Hedgehog signalling plays key roles in embryogenesis and adult tissue homeostasis, and is central to skeletal development and the osteogenic differentiation of mesenchymal stem cells. The expression of several components of the Hedgehog signalling pathway have been reported to be mechanically regulated in mesodermal tissue patterning and osteogenic differentiation in response to external stimulation. Since a number of bone developmental defects and skeletal diseases, such as osteoporosis, are directly linked to aberrant Hedgehog signalling, a better knowledge of the regulation of Hedgehog signalling in the mechanosensitive bone marrow-residing mesenchymal stromal cells will present novel avenues for modelling these diseases and uncover novel opportunities for extracellular matrix-targeted therapies. In this review, we present a brief overview of the key molecular players involved in Hedgehog signalling and the basic concepts of mechanobiology, with a focus on bone development and regeneration. We also highlight the correlation between the activation of the Hedgehog signalling pathway in response to mechanical cues and osteogenesis in bone marrow-derived mesenchymal stromal cells. Finally, we propose different tissue engineering strategies to apply the expanding knowledge of 3D material-cell interactions in the modulation of Hedgehog signalling in vitro for fundamental and translational research applications.

Original languageEnglish
Article number100502
Pages (from-to)1-17
Number of pages17
JournalMaterials Today Bio
Volume17
Early online date28 Nov 2022
DOIs
Publication statusPublished - 15 Dec 2022

Keywords

  • extracellular matrix
  • hedgehog signalling
  • mechanobiology
  • mechanotransduction
  • mesenchymal stromal cells
  • osteogenesis

Fingerprint

Dive into the research topics of 'From mesenchymal niches to engineered in vitro model systems: Exploring and exploiting biomechanical regulation of vertebrate hedgehog signalling'. Together they form a unique fingerprint.

Cite this