Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical

Carlos Fernández-de-Alba; Hiroki Takahashi; Alexandre Richard; Yves Chenavier; Lionel Dubois; Vincent Maurel; Daniel Lee; Sabine Hediger; Gaël De Paëpe

doi:10.1002/chem.201404588

Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical

Carlos Fernández-de-Alba, Hiroki Takahashi, Alexandre Richard, Yves Chenavier, Lionel Dubois, Vincent Maurel, Daniel Lee, Sabine Hediger, Gaël De Paëpe^*

^*Corresponding author for this work

CE - Academic & Research

Research output: Contribution to journal › Article › peer-review

Abstract

Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.

Original language	English
Pages (from-to)	4512-4517
Number of pages	6
Journal	Chemistry - A European Journal
Volume	21
Issue number	12
Early online date	6 Feb 2015
DOIs	https://doi.org/10.1002/chem.201404588
Publication status	Published - 16 Mar 2015

Keywords

dynamic nuclear polarization
membranes
NMR spectroscopy
phospholipids
solid-state NMR spectroscopy

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title = "Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical",

abstract = "Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.",

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AU - Fernández-de-Alba, Carlos

AU - Takahashi, Hiroki

AU - Richard, Alexandre

AU - Chenavier, Yves

AU - Dubois, Lionel

AU - Maurel, Vincent

AU - Lee, Daniel

AU - Hediger, Sabine

AU - De Paëpe, Gaël

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N2 - Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.

AB - Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.

KW - dynamic nuclear polarization

KW - membranes

KW - NMR spectroscopy

KW - phospholipids

KW - solid-state NMR spectroscopy

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Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical

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Keywords

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