Functional analysis of CFTR chloride channel activity in cells with elevated MDR1 expression

Lishuang Cao; Grzegorz Owsianik; Martine Jaspers; Annelies Janssens; Harry Cuppens; Jean Jacques Cassiman; Bernd Nilius

doi:10.1016/S0006-291X(03)00581-3

Functional analysis of CFTR chloride channel activity in cells with elevated MDR1 expression

Lishuang Cao, Grzegorz Owsianik, Martine Jaspers, Annelies Janssens, Harry Cuppens, Jean Jacques Cassiman, Bernd Nilius

Research output: Contribution to journal › Article › peer-review

Abstract

Using the patch-clamp method, we investigated a relationship between MDR1 expression and its effects on the CFTR channel function. Incubation of CaCo-2 cells with increasing concentrations of doxorubicin resulted in a reduction of CFTR chloride channel activity in a dose-dependent manner. This reduction was associated with a decrease of CFTR mRNA and simultaneous up-regulation of MDR1 mRNA in the presence of doxorubicin. Similar alteration of the CFTR function was observed in CaCo-2 cells transiently overexpressing MDR1. No alterations of the cAMP-dependent chloride currents were observed in COS-1 cells transiently co-expressing CFTR and MDR1 from strong CMV promoters. This indicated that repression of CFTR by MDR1 induction requires the presence of the native CFTR promoter. © 2003 Elsevier Science (USA). All rights reserved.

Original language	English
Pages (from-to)	248-252
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	304
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(03)00581-3
Publication status	Published - 2 May 2003

Keywords

cAMP-activated chloride channel
CFTR
Co-expression
Cystic fibrosis
Doxorubicin
MDR1
Multidrug resistance
Patch-clamp technique

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10.1016/S0006-291X(03)00581-3

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title = "Functional analysis of CFTR chloride channel activity in cells with elevated MDR1 expression",

abstract = "Using the patch-clamp method, we investigated a relationship between MDR1 expression and its effects on the CFTR channel function. Incubation of CaCo-2 cells with increasing concentrations of doxorubicin resulted in a reduction of CFTR chloride channel activity in a dose-dependent manner. This reduction was associated with a decrease of CFTR mRNA and simultaneous up-regulation of MDR1 mRNA in the presence of doxorubicin. Similar alteration of the CFTR function was observed in CaCo-2 cells transiently overexpressing MDR1. No alterations of the cAMP-dependent chloride currents were observed in COS-1 cells transiently co-expressing CFTR and MDR1 from strong CMV promoters. This indicated that repression of CFTR by MDR1 induction requires the presence of the native CFTR promoter. {\textcopyright} 2003 Elsevier Science (USA). All rights reserved.",

keywords = "cAMP-activated chloride channel, CFTR, Co-expression, Cystic fibrosis, Doxorubicin, MDR1, Multidrug resistance, Patch-clamp technique",

author = "Lishuang Cao and Grzegorz Owsianik and Martine Jaspers and Annelies Janssens and Harry Cuppens and Cassiman, \{Jean Jacques\} and Bernd Nilius",

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T1 - Functional analysis of CFTR chloride channel activity in cells with elevated MDR1 expression

AU - Cao, Lishuang

AU - Owsianik, Grzegorz

AU - Jaspers, Martine

AU - Janssens, Annelies

AU - Cuppens, Harry

AU - Cassiman, Jean Jacques

AU - Nilius, Bernd

PY - 2003/5/2

Y1 - 2003/5/2

N2 - Using the patch-clamp method, we investigated a relationship between MDR1 expression and its effects on the CFTR channel function. Incubation of CaCo-2 cells with increasing concentrations of doxorubicin resulted in a reduction of CFTR chloride channel activity in a dose-dependent manner. This reduction was associated with a decrease of CFTR mRNA and simultaneous up-regulation of MDR1 mRNA in the presence of doxorubicin. Similar alteration of the CFTR function was observed in CaCo-2 cells transiently overexpressing MDR1. No alterations of the cAMP-dependent chloride currents were observed in COS-1 cells transiently co-expressing CFTR and MDR1 from strong CMV promoters. This indicated that repression of CFTR by MDR1 induction requires the presence of the native CFTR promoter. © 2003 Elsevier Science (USA). All rights reserved.

AB - Using the patch-clamp method, we investigated a relationship between MDR1 expression and its effects on the CFTR channel function. Incubation of CaCo-2 cells with increasing concentrations of doxorubicin resulted in a reduction of CFTR chloride channel activity in a dose-dependent manner. This reduction was associated with a decrease of CFTR mRNA and simultaneous up-regulation of MDR1 mRNA in the presence of doxorubicin. Similar alteration of the CFTR function was observed in CaCo-2 cells transiently overexpressing MDR1. No alterations of the cAMP-dependent chloride currents were observed in COS-1 cells transiently co-expressing CFTR and MDR1 from strong CMV promoters. This indicated that repression of CFTR by MDR1 induction requires the presence of the native CFTR promoter. © 2003 Elsevier Science (USA). All rights reserved.

KW - cAMP-activated chloride channel

KW - CFTR

KW - Co-expression

KW - Cystic fibrosis

KW - Doxorubicin

KW - MDR1

KW - Multidrug resistance

KW - Patch-clamp technique

UR - https://www.scopus.com/pages/publications/0037414432

U2 - 10.1016/S0006-291X(03)00581-3

DO - 10.1016/S0006-291X(03)00581-3

M3 - Article

SN - 1090-2104

VL - 304

SP - 248

EP - 252

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Functional analysis of CFTR chloride channel activity in cells with elevated MDR1 expression

Abstract

Keywords

UN SDGs

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