Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

M. F. Kiernan; A. Barrie; J. Szkolar; T. A. Mills; M. Wareing

doi:10.1016/j.placenta.2010.03.009

Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

M. F. Kiernan, A. Barrie, J. Szkolar, T. A. Mills, M. Wareing

Research output: Contribution to journal › Article › peer-review

Abstract

Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P <0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively. © 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	553-555
Number of pages	2
Journal	Placenta
Volume	31
Issue number	6
DOIs	https://doi.org/10.1016/j.placenta.2010.03.009
Publication status	Published - Jun 2010

Keywords

Human
Placenta vasculature
Potassium channel

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10.1016/j.placenta.2010.03.009

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title = "Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature",

abstract = "Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P <0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively. {\textcopyright} 2010 Elsevier Ltd. All rights reserved.",

keywords = "Human, Placenta vasculature, Potassium channel",

author = "Kiernan, {M. F.} and A. Barrie and J. Szkolar and Mills, {T. A.} and M. Wareing",

year = "2010",

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doi = "10.1016/j.placenta.2010.03.009",

language = "English",

volume = "31",

pages = "553--555",

journal = "Placenta",

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TY - JOUR

T1 - Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

AU - Kiernan, M. F.

AU - Barrie, A.

AU - Szkolar, J.

AU - Mills, T. A.

AU - Wareing, M.

PY - 2010/6

Y1 - 2010/6

N2 - Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P <0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively. © 2010 Elsevier Ltd. All rights reserved.

AB - Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P <0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively. © 2010 Elsevier Ltd. All rights reserved.

KW - Human

KW - Placenta vasculature

KW - Potassium channel

U2 - 10.1016/j.placenta.2010.03.009

DO - 10.1016/j.placenta.2010.03.009

M3 - Article

SN - 0143-4004

VL - 31

SP - 553

EP - 555

JO - Placenta

JF - Placenta

IS - 6

ER -

Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

Abstract

Keywords

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