Fungal succession in an in-vessel composting system characterized using 454 pyrosequencing

Adrian Langarica-Fuentes, Urooj Zafar, Alan Heyworth, Thomas Brown, Graeme Fox, Geoffrey D. Robson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Fungi are known to have an important role in the composting process as degraders of recalcitrant materials such as cellulose and lignin. Previous attempts to study the diversity and succession of fungi in compost systems have relied on the use of culture-dependent analyses and low-resolution DNA-fingerprinting techniques, lacking the necessary depth to analyse such a rich ecosystem. In this study, 454 pyrosequencing was used to characterize the fungal community composition at the different stages of an in-vessel composting process. A complex succession of fungi was revealed, with 251 fungal OTUs identified throughout the monitoring period. The Ascomycota were the dominant phylum (82.5% of all sequences recovered), followed by the Basidiomycota (10.4%) and the subphylum Mucoromycotina (4.9%). In the starting materials and early stages of the process, yeast species from the Saccharomycetales were abundant, while in latter stages and in the high temperature regions of the pile, fungi from the orders Eurotiales, Sordariales, Mucorales, Agaricales and Microascales were the most prominent. This study provides an improved understanding of the fungal diversity occurring during the composting of municipal solid waste, and this knowledge can lead to the development of more efficient composting practices and a better evaluation of the end-product quality. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)296-308
    Number of pages12
    JournalFEMS Microbiology Ecology
    Volume88
    Issue number2
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Biodiversity
    • Compost
    • Thermophiles

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