Further evaluation of the local lymph node assay in the final phase of an international collaborative trial

Scott E. Loveless, Gregory S. Ladics, G. Frank Gerberick, Cindy A. Ryan, David A. Basketter, Edward W. Scholes, Robert V. House, Jennifer Hilton, Rebecca J. Dearman, Ian Kimber

    Research output: Contribution to journalArticlepeer-review


    The local lymph node assay (LLNA) is a method used for the prospective identification in mice of chemicals that have the potential to cause skin sensitization. We report here the results of the second and final phase of an international trial in which the performance of the assay has been evaluated using seven test materials in five independent laboratories. The additional chemicals examined here included compounds which are considered less potent allergens than some of those tested in the first phase of the investigation, and includes hexylcinnamic aldehyde (HCA), a chemical recommended by the Organization for Economic Cooperation and Development (OECD) as a positive control for skin sensitization studies. In each laboratory all skin sensitizing chemicals examined (2,4-dinitrochlorobenzene 1 DNCB 1, HCA, oxazolone, isoeugenol and eugenol) elicited positive responses of comparable magnitude as judged by the derived lowest concentration of test chemical required to elicit a 3-fold or greater increase in the proliferative activity of draining lymph node cells compared with vehicle-treated controls. We observed that sodium lauryl sulphate, considered to be a non-sensitizing skin irritant, also induced a positive response in the assay. Para-aminobenzoic acid (pABA), a nonsensitizing chemical, was negative at all test concentrations in each laboratory. Some laboratories incorporated minor modifications into the standard assay procedure, including the evaluation of lymph nodes pooled from individual mice rather than treatment groups and the use of statistical analyses. The use of statistics did not markedly change the determination of the lowest concentration yielding a positive response. These data confirm that the local lymph node assay is robust and yields equivalent results when performed independently.
    Original languageEnglish
    Pages (from-to)141-152
    Number of pages11
    Issue number1-2
    Publication statusPublished - 15 Apr 1996


    • Chemical allergens
    • Hypersensitivity
    • Local lymph node assay
    • Predictive testing
    • Skin sensitization


    Dive into the research topics of 'Further evaluation of the local lymph node assay in the final phase of an international collaborative trial'. Together they form a unique fingerprint.

    Cite this