GABA mediates autoreceptor feedback inhibition in the rat carotid body via presynaptic GABAB receptors and TASK-1

Ian M. Fearon, Min Zhang, Cathy Vollmer, Colin A. Nurse

    Research output: Contribution to journalArticlepeer-review


    Background K+ channels exert control over neuronal excitability by regulating resting potential and input resistance. Here, we show that GABAB receptor-mediated activation of a background K+ conductance modulates transmission at rat carotid body chemosensory synapses in vitro. Carotid body chemoreceptor (type I) cells expressed GABAB(1) and GABAB(2) subunits as well as endogenous GABA. The GABAB receptor agonist baclofen activated an anandamide-and Ba2+-sensitive TASK-1-like background K+ conductance in chemoreceptor cell clusters, but was without effect on voltagegated Ca2+ channels. Hydroxysaclofen (50 μM), 5-aminovaleric acid (100 μM) and CGP 55845 (100 nM), selective GABAB receptor blockers, potentiated the hypoxia-induced receptor potential; this effect was abolished by pre-treatment with pertussis toxin (PTX; 500 ng ml-1), an inhibitor of Gi, or by H-89 (50 μM), a selective inhibitor of protein kinase A. The protein kinase C inhibitor chelerythrine chloride (100 μM) was without effect on this potentiation. GABAB receptor blockers also caused depolarisation of type I cells in clusters, and enhahced spike discharge in spontaneously firing cells. In functional co-cultures of type I clusters and petrosal sensory neurones, GABAB receptor blockers potentiated hypoxia-induced postsynaptic chemosensory responses mediated by the fast-acting transmitters ACh and ATP. Thus GABAB receptor-mediated activation of TASK-1 or a related channel provides a presynaptic autoregulatory feedback mechanism that modulates fast synaptic transmission in the rat carotid body.
    Original languageEnglish
    Pages (from-to)83-94
    Number of pages11
    JournalJournal of Physiology
    Issue number1
    Publication statusPublished - 15 Nov 2003


    Dive into the research topics of 'GABA mediates autoreceptor feedback inhibition in the rat carotid body via presynaptic GABAB receptors and TASK-1'. Together they form a unique fingerprint.

    Cite this