Gas exchange measurements for the determination of photosynthetic efficiency in Arabidopsis leaves

Giles Johnson, Erik Murchie

    Research output: Chapter in Book/Conference proceedingChapter

    Abstract

    Photosynthesis is one of the most readily measured metabolic processes in a plant, with fluxes being measurable non-invasively even under field conditions. In this chapter, two principal approaches are described to measure photosynthesis - O 2 evolution as determined using an O 2 electrode, and CO 2 fixation which can be quantified using an infrared gas analyser. The advantages and disadvantages of these different methods, as applied to Arabidopsis, are discussed, and some major forms of analysis are described. © 2011 Springer Science+Business Media, LLC.
    Original languageEnglish
    Title of host publicationMethods in Molecular Biology|Methods Mol. Biol.
    PublisherHumana Press, Inc
    Pages311-326
    Number of pages15
    Volume775
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Assimilation
    • CO 2 response curve
    • Gas exchange
    • Light response curve
    • Photosynthesis

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