Gastritis induced by Helicobacter pylori infection in experimental rats

Mohamed M. Elseweidy; Mona M. Taha; Nahla N. Younis; Khadiga S. Ibrahim; Hamdi A. Hamouda; Mohamed A. Eldosouky; Hala Soliman

doi:10.1007/s10620-009-1103-y

Gastritis induced by Helicobacter pylori infection in experimental rats

Mohamed M. Elseweidy, Mona M. Taha, Nahla N. Younis, Khadiga S. Ibrahim, Hamdi A. Hamouda, Mohamed A. Eldosouky, Hala Soliman

Research output: Contribution to journal › Article › peer-review

Abstract

Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-yearold male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E2 (PGE2) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE2 demonstrated significant increases joined with a decreased serum pepsinogen I activity (P

Original language	English
Pages (from-to)	2770-2777
Number of pages	7
Journal	Digestive Diseases and Sciences
Volume	55
Issue number	10
DOIs	https://doi.org/10.1007/s10620-009-1103-y
Publication status	Published - Oct 2010

Keywords

DNA fragmentation
Experimental H. pylori gastritis
Gastrin
INOS
Myeloperoxidase
Pepsinogen
Prostaglandin E2
Rat model

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title = "Gastritis induced by Helicobacter pylori infection in experimental rats",

abstract = "Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-yearold male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E2 (PGE2) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE2 demonstrated significant increases joined with a decreased serum pepsinogen I activity (P",

keywords = "DNA fragmentation, Experimental H. pylori gastritis, Gastrin, INOS, Myeloperoxidase, Pepsinogen, Prostaglandin E2, Rat model",

author = "Elseweidy, {Mohamed M.} and Taha, {Mona M.} and Younis, {Nahla N.} and Ibrahim, {Khadiga S.} and Hamouda, {Hamdi A.} and Eldosouky, {Mohamed A.} and Hala Soliman",

year = "2010",

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doi = "10.1007/s10620-009-1103-y",

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T1 - Gastritis induced by Helicobacter pylori infection in experimental rats

AU - Elseweidy, Mohamed M.

AU - Taha, Mona M.

AU - Younis, Nahla N.

AU - Ibrahim, Khadiga S.

AU - Hamouda, Hamdi A.

AU - Eldosouky, Mohamed A.

AU - Soliman, Hala

PY - 2010/10

Y1 - 2010/10

N2 - Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-yearold male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E2 (PGE2) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE2 demonstrated significant increases joined with a decreased serum pepsinogen I activity (P

AB - Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-yearold male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E2 (PGE2) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE2 demonstrated significant increases joined with a decreased serum pepsinogen I activity (P

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KW - Experimental H. pylori gastritis

KW - Gastrin

KW - INOS

KW - Myeloperoxidase

KW - Pepsinogen

KW - Prostaglandin E2

KW - Rat model

U2 - 10.1007/s10620-009-1103-y

DO - 10.1007/s10620-009-1103-y

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EP - 2777

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 10

ER -

Gastritis induced by Helicobacter pylori infection in experimental rats

Abstract

Keywords

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