Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos

Brian Stramer, Mark Winfield, Tanya Shaw, Thomas H. Millard, Sarah Woolner, Paul Martin

    Research output: Contribution to journalArticlepeer-review

    Abstract

    By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.
    Original languageEnglish
    Pages (from-to)465-471
    Number of pages6
    JournalEMBO reports
    Volume9
    Issue number5
    DOIs
    Publication statusPublished - May 2008

    Fingerprint

    Dive into the research topics of 'Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos'. Together they form a unique fingerprint.

    Cite this