@article{c99d9a479d6940b788e34ad837213ceb,
title = "Gene-Specific Linear Trends Constrain Transcriptional Variability of the Toll-like Receptor Signaling",
abstract = "This study demonstrates that transcriptional variability of the toll-like receptor signaling is constrained by mean mRNA responses through gene-specific modulation of transcriptional bursting.",
keywords = "IL-1β, TNF-α, cellular heterogeneity, single-cell transcriptomics, stochastic gene expression, stochastic modeling, toll-like receptor, transcriptional bursting",
author = "James Bagnall and William Rowe and Nissrin Alachkar and James Roberts and Hazel England and Christopher Clark and Mark Platt and Dean Jackson and Mark Muldoon and Pawel Paszek",
note = "Funding Information: We thank David Spiller and other members of the Systems Microscopy Centre in Manchester for discussions. We also thank Stuart Pepper for help with the Fluidigm system and the Bioimaging and Genomic Technologies Core Facilities in the Faculty of Biology, Medicine and Health in Manchester for assistance. We also thank Dale Norton for commenting on the manuscript as well as Conor Clerkin-Oliver, Ran Duan, Xinye Han, Zenthian Kai, Harriet Ninsiima, Stephen Richter, Dessislava Veltcheva, and Toby Wilkinson for the help with genomics analyses. This work was supported by BBSRC ( BB/I017976/1 , BB/K003097/1 , and BB/R007691/1 ); Wellcome Trust ( 097820/Z/11/B and 220025/Z/19/Z ); and by the National Science Center, Poland ( https://ncn.gov.pl ) grant 2016/23/B/ST6/03455 . The work has received funding from the European Union Seventh Framework Programme ( FP7/2012-2017 ) under grant agreement no. 305564. Funding Information: We thank David Spiller and other members of the Systems Microscopy Centre in Manchester for discussions. We also thank Stuart Pepper for help with the Fluidigm system and the Bioimaging and Genomic Technologies Core Facilities in the Faculty of Biology, Medicine and Health in Manchester for assistance. We also thank Dale Norton for commenting on the manuscript as well as Conor Clerkin-Oliver, Ran Duan, Xinye Han, Zenthian Kai, Harriet Ninsiima, Stephen Richter, Dessislava Veltcheva, and Toby Wilkinson for the help with genomics analyses. This work was supported by BBSRC (BB/I017976/1, BB/K003097/1, and BB/R007691/1); Wellcome Trust (097820/Z/11/B and 220025/Z/19/Z); and by the National Science Center, Poland (https://ncn.gov.pl) grant 2016/23/B/ST6/03455. The work has received funding from the European Union Seventh Framework Programme (FP7/2012-2017) under grant agreement no. 305564. Conceptualization, P.P. J.B. W.R. and M.M.; Methodology, J.B. W.R. N.A. C.C. M.M. and P.P.; Formal Analysis, J.B. W.R. N.A. J.R. and P.P.; Investigation, J.B. W.R. N.A. J.R. C.C. and P.P.; Resources, H.E.; Data Curation: J.B. and W.R.; Writing – Original Draft, P.P. Writing – Review & Editing, J.B. W.R. D.J. and M.M.; Visualization, J.B. W.R. N.A. and P.P.; Project administration, P.P.; Funding Acquisition, P.P. D.J. and M.M.; Supervision, M.P. M.M. and P.P. The authors declare no conflict of interests. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = sep,
day = "23",
doi = "10.1016/j.cels.2020.08.007",
language = "English",
volume = "11",
pages = "300--314.e8",
journal = "Cell Systems",
issn = "2405-4712",
publisher = "Cell Press",
number = "3",
}