Genetic and physical mapping around the properdin P gene

M. P. Coleman; J. C. Murray; H. F. Willard; K. F. Nolan; K. B M Reid; D. J. Blake; S. Lindsay; S. S. Bhattacharya; A. Wright; K. E. Davies

Genetic and physical mapping around the properdin P gene

M. P. Coleman, J. C. Murray, H. F. Willard, K. F. Nolan, K. B M Reid, D. J. Blake, S. Lindsay, S. S. Bhattacharya, A. Wright, K. E. Davies

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Abstract

A CA repeat has been found on the human X chromosome within 16 kb of the gene encoding properdin P factor (PFC) and has been shown to be a highly informative marker. Two more polymorphic CA repeats were found in a cosmid containing DXS228. The CA repeats, and other markers from proximal Xp, were mapped genetically in CEPH families and the likely order of markers was established as Xpter-(DXS7, MAO-A, DXS228)-(PFC, DXS426)-(TIMP, OATL1)-DXS255-Xcen. This places PFC in the region Xp11.3-Xp11.23, thus refining previous in situ hybridization data. Two yeast artificial chromosomes (YACs) (440 and 390 kb) contain both PFC and DXS426, and one of them (440 kb) also contains TIMP. This confirms the genetic order TIMP-(PFC, DXS426). PFC and TIMP are located on the same 100-kb SalI PvuI fragment of the 440-kb YAC. Given the genetic orientation of TIMP and (PFC, DXS426), this YAC can now serve as a starting point for directional walking toward disease genes located in Xp11.3-Xp11.2 such as retinitis pigmentosa (RP2) and Wiskott-Aldrich syndrome. © 1991.

Original language	English
Pages (from-to)	991-996
Number of pages	5
Journal	Genomics
Volume	11
Issue number	4
Publication status	Published - Dec 1991

Keywords

Base Sequence
Centromere
Chromosome Mapping
Chromosomes, Fungal
Cloning, Molecular
Dna
Female
Genetic Linkage
Genetic Markers/genetics
Genome, Human
Humans
Male
Molecular Sequence Data
Pedigree
Properdin/*genetics
Repetitive Sequences, Nucleic Acid

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@article{86c6210f16a84435bfab78baff2321c9,

title = "Genetic and physical mapping around the properdin P gene",

abstract = "A CA repeat has been found on the human X chromosome within 16 kb of the gene encoding properdin P factor (PFC) and has been shown to be a highly informative marker. Two more polymorphic CA repeats were found in a cosmid containing DXS228. The CA repeats, and other markers from proximal Xp, were mapped genetically in CEPH families and the likely order of markers was established as Xpter-(DXS7, MAO-A, DXS228)-(PFC, DXS426)-(TIMP, OATL1)-DXS255-Xcen. This places PFC in the region Xp11.3-Xp11.23, thus refining previous in situ hybridization data. Two yeast artificial chromosomes (YACs) (440 and 390 kb) contain both PFC and DXS426, and one of them (440 kb) also contains TIMP. This confirms the genetic order TIMP-(PFC, DXS426). PFC and TIMP are located on the same 100-kb SalI PvuI fragment of the 440-kb YAC. Given the genetic orientation of TIMP and (PFC, DXS426), this YAC can now serve as a starting point for directional walking toward disease genes located in Xp11.3-Xp11.2 such as retinitis pigmentosa (RP2) and Wiskott-Aldrich syndrome. {\textcopyright} 1991.",

keywords = "Base Sequence, Centromere, Chromosome Mapping, Chromosomes, Fungal, Cloning, Molecular, Dna, Female, Genetic Linkage, Genetic Markers/genetics, Genome, Human, Humans, Male, Molecular Sequence Data, Pedigree, Properdin/*genetics, Repetitive Sequences, Nucleic Acid",

author = "Coleman, {M. P.} and Murray, {J. C.} and Willard, {H. F.} and Nolan, {K. F.} and Reid, {K. B M} and Blake, {D. J.} and S. Lindsay and Bhattacharya, {S. S.} and A. Wright and Davies, {K. E.}",

note = "DE08556/DE/NIDCR NIH HHS/United States GM40864/GM/NIGMS NIH HHS/United States Wellcome Trust/United Kingdom Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. United states",

year = "1991",

month = dec,

language = "English",

volume = "11",

pages = "991--996",

journal = "Genomics",

issn = "0888-7543",

publisher = "Elsevier BV",

number = "4",

}

TY - JOUR

T1 - Genetic and physical mapping around the properdin P gene

AU - Coleman, M. P.

AU - Murray, J. C.

AU - Willard, H. F.

AU - Nolan, K. F.

AU - Reid, K. B M

AU - Blake, D. J.

AU - Lindsay, S.

AU - Bhattacharya, S. S.

AU - Wright, A.

AU - Davies, K. E.

N1 - DE08556/DE/NIDCR NIH HHS/United States GM40864/GM/NIGMS NIH HHS/United States Wellcome Trust/United Kingdom Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. United states

PY - 1991/12

Y1 - 1991/12

N2 - A CA repeat has been found on the human X chromosome within 16 kb of the gene encoding properdin P factor (PFC) and has been shown to be a highly informative marker. Two more polymorphic CA repeats were found in a cosmid containing DXS228. The CA repeats, and other markers from proximal Xp, were mapped genetically in CEPH families and the likely order of markers was established as Xpter-(DXS7, MAO-A, DXS228)-(PFC, DXS426)-(TIMP, OATL1)-DXS255-Xcen. This places PFC in the region Xp11.3-Xp11.23, thus refining previous in situ hybridization data. Two yeast artificial chromosomes (YACs) (440 and 390 kb) contain both PFC and DXS426, and one of them (440 kb) also contains TIMP. This confirms the genetic order TIMP-(PFC, DXS426). PFC and TIMP are located on the same 100-kb SalI PvuI fragment of the 440-kb YAC. Given the genetic orientation of TIMP and (PFC, DXS426), this YAC can now serve as a starting point for directional walking toward disease genes located in Xp11.3-Xp11.2 such as retinitis pigmentosa (RP2) and Wiskott-Aldrich syndrome. © 1991.

AB - A CA repeat has been found on the human X chromosome within 16 kb of the gene encoding properdin P factor (PFC) and has been shown to be a highly informative marker. Two more polymorphic CA repeats were found in a cosmid containing DXS228. The CA repeats, and other markers from proximal Xp, were mapped genetically in CEPH families and the likely order of markers was established as Xpter-(DXS7, MAO-A, DXS228)-(PFC, DXS426)-(TIMP, OATL1)-DXS255-Xcen. This places PFC in the region Xp11.3-Xp11.23, thus refining previous in situ hybridization data. Two yeast artificial chromosomes (YACs) (440 and 390 kb) contain both PFC and DXS426, and one of them (440 kb) also contains TIMP. This confirms the genetic order TIMP-(PFC, DXS426). PFC and TIMP are located on the same 100-kb SalI PvuI fragment of the 440-kb YAC. Given the genetic orientation of TIMP and (PFC, DXS426), this YAC can now serve as a starting point for directional walking toward disease genes located in Xp11.3-Xp11.2 such as retinitis pigmentosa (RP2) and Wiskott-Aldrich syndrome. © 1991.

KW - Base Sequence

KW - Centromere

KW - Chromosome Mapping

KW - Chromosomes, Fungal

KW - Cloning, Molecular

KW - Dna

KW - Female

KW - Genetic Linkage

KW - Genetic Markers/genetics

KW - Genome, Human

KW - Humans

KW - Male

KW - Molecular Sequence Data

KW - Pedigree

KW - Properdin/genetics

KW - Repetitive Sequences, Nucleic Acid

M3 - Article

SN - 0888-7543

VL - 11

SP - 991

EP - 996

JO - Genomics

JF - Genomics

IS - 4

ER -

Genetic and physical mapping around the properdin P gene

Abstract

Keywords

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