Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

T. M. Skinner; N. L. Lopez-Corrales; S. I. Anderson; A. S I Loudon; S. Lowden; C. S. Haley; A. L. Archibald

doi:10.1159/000057022

Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

T. M. Skinner
, N. L. Lopez-Corrales
, S. I. Anderson
, A. S I Loudon
, S. Lowden
, C. S. Haley
, A. L. Archibald

Research output: Contribution to journal › Article › peer-review

Abstract

The porcine PER1 gene was mapped to chromosome 12q1.4→q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue. Copyright © 2002 S. Karger AG, Basel.

Original language	English
Pages (from-to)	82-84
Number of pages	2
Journal	Cytogenetics and Cell Genetics
Volume	95
Issue number	1-2
DOIs	https://doi.org/10.1159/000057022
Publication status	Published - 2001

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title = "Genetic and physical mapping, expression analysis and partial sequence of porcine PER1",

abstract = "The porcine PER1 gene was mapped to chromosome 12q1.4→q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96\% identity with its human orthologue. Copyright {\textcopyright} 2002 S. Karger AG, Basel.",

author = "Skinner, \{T. M.\} and Lopez-Corrales, \{N. L.\} and Anderson, \{S. I.\} and Loudon, \{A. S I\} and S. Lowden and Haley, \{C. S.\} and Archibald, \{A. L.\}",

year = "2001",

doi = "10.1159/000057022",

language = "English",

volume = "95",

pages = "82--84",

journal = "Cytogenetics and Cell Genetics",

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publisher = "S. Karger AG",

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TY - JOUR

T1 - Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

AU - Skinner, T. M.

AU - Lopez-Corrales, N. L.

AU - Anderson, S. I.

AU - Loudon, A. S I

AU - Lowden, S.

AU - Haley, C. S.

AU - Archibald, A. L.

PY - 2001

Y1 - 2001

N2 - The porcine PER1 gene was mapped to chromosome 12q1.4→q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue. Copyright © 2002 S. Karger AG, Basel.

AB - The porcine PER1 gene was mapped to chromosome 12q1.4→q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue. Copyright © 2002 S. Karger AG, Basel.

U2 - 10.1159/000057022

DO - 10.1159/000057022

M3 - Article

C2 - 11978975

SN - 0301-0171

VL - 95

SP - 82

EP - 84

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

IS - 1-2

ER -

Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

Abstract

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