Genetic basis of aminoglycoside resistance following changes in aminoglycoside prescription patterns

Chris Kosmidis, Maria Giannopoulou, Anastasia Flountzi, Antonis Markogiannakis, Dimitris Goukos, George Petrikkos, George L Daikos, Konstantina Tzanetou

Research output: Contribution to journalArticlepeer-review


Aminoglycosides (AG) offer an important therapeutic option for the treatment of infections caused by multiresistant Enterobacteriaceae. We observed a change in AG usage patterns in our institution between 1997 and 2006, namely a reduction in use of all AG except amikacin. We studied the changes in AG susceptibility rates in these time periods and correlated with prevalence of different molecular resistance mechanisms. Enterobacteriaceae isolated from blood cultures from 1997 and 2006 were studied. Susceptibilities to AG were determined with the disk diffusion method. PCR was used to detect genes encoding AG-modifying enzymes and methylases. Gentamicin resistance rates dropped from 14·5 to 8·8%, whereas resistance rates to other AG remained unchanged. The AAC(6')-I+AAC(3)-I combination was more common in 1997, whereas AAC(6')-I was the most common mechanism in 2006. Reduction in gentamicin use may preserve the usefulness of this agent against severe infections by multiresistant bacteria such as carbapenemase-producing Enterobacteriaceae.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalJournal of chemotherapy (Florence, Italy)
Issue number4
Publication statusPublished - Aug 2013


  • Amikacin/pharmacology
  • Aminoglycosides/pharmacology
  • Anti-Bacterial Agents/pharmacology
  • Drug Resistance, Multiple/genetics
  • Drug Utilization
  • Enterobacteriaceae/drug effects
  • Enterobacteriaceae Infections/drug therapy
  • Genotype
  • Gentamicins/pharmacology
  • Greece/epidemiology
  • Humans
  • Microbial Sensitivity Tests
  • Practice Patterns, Physicians'


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