Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor

L B John; T M Chee; D E Gilham; P K Darcy

doi:10.1007/978-1-4939-0345-0_16

Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor

L B John, T M Chee, D E Gilham, P K Darcy

Research output: Contribution to journal › Article › peer-review

Abstract

Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.

Original language	English
Pages (from-to)	177-87
Number of pages	89
Journal	Methods Mol Biol
Volume	1139
DOIs	https://doi.org/10.1007/978-1-4939-0345-0_16
Publication status	Published - 2014

Keywords

Animals
CD8-Positive T-Lymphocytes/cytology/immunology/*metabolism
Cell Separation
Cell Survival
Cytokines/secretion
Gene Expression
Genetic Engineering/*methods
Immunotherapy, Adoptive
Mice
Receptors, Antigen/*genetics/immunology/metabolism
Recombinant Fusion Proteins/*genetics
Retroviridae/genetics
Spleen/cytology
T-Lymphocytes, Helper-Inducer/immunology/*metabolism
Transduction, Genetic

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10.1007/978-1-4939-0345-0_16

http://www.ncbi.nlm.nih.gov/pubmed/24619680

Cite this

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title = "Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor",

abstract = "Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.",

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author = "John, {L B} and Chee, {T M} and Gilham, {D E} and Darcy, {P K}",

note = "John, Liza B Chee, Tess M Gilham, David E Darcy, Phillip K eng Clifton, N.J. 2014/03/13 06:00 Methods Mol Biol. 2014;1139:177-87. doi: 10.1007/978-1-4939-0345-0_16.",

year = "2014",

doi = "10.1007/978-1-4939-0345-0_16",

language = "English",

volume = "1139",

pages = "177--87",

journal = "Methods Mol Biol",

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T1 - Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor

AU - John, L B

AU - Chee, T M

AU - Gilham, D E

AU - Darcy, P K

N1 - John, Liza B Chee, Tess M Gilham, David E Darcy, Phillip K eng Clifton, N.J. 2014/03/13 06:00 Methods Mol Biol. 2014;1139:177-87. doi: 10.1007/978-1-4939-0345-0_16.

PY - 2014

Y1 - 2014

N2 - Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.

AB - Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.

KW - Animals

KW - CD8-Positive T-Lymphocytes/cytology/immunology/metabolism

KW - Cell Separation

KW - Cell Survival

KW - Cytokines/secretion

KW - Gene Expression

KW - Genetic Engineering/methods

KW - Immunotherapy, Adoptive

KW - Mice

KW - Receptors, Antigen/genetics/immunology/metabolism

KW - Recombinant Fusion Proteins/genetics

KW - Retroviridae/genetics

KW - Spleen/cytology

KW - T-Lymphocytes, Helper-Inducer/immunology/metabolism

KW - Transduction, Genetic

U2 - 10.1007/978-1-4939-0345-0_16

DO - 10.1007/978-1-4939-0345-0_16

M3 - Article

SN - 1940-6029

VL - 1139

SP - 177

EP - 187

JO - Methods Mol Biol

JF - Methods Mol Biol

ER -

Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor

Abstract

Keywords

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