Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding

Catharine West, Rachel E. Airley, Juliette Loncaster, James A. Raleigh, Adrian L. Harris, Susan E. Davidson, Robert D. Hunter, Catharine M L West, Ian J. Stratford

    Research output: Contribution to journalArticlepeer-review


    The presence of hypoxia in tumours results in the overexpression of certain genes, which are controlled via the transcription factor HIF-1. Hypoxic cells are known to be radioresistant and chemoresistant, thus, a reliable surrogate marker of hypoxia is desirable to ensure that treatment may be rationally applied. Recently, the HIF-1-regulated proteins Glut-1 and CAIX were validated as intrinsic markers of hypoxia by comparison with pO2 measured using oxygen electrodes. We compare the expression of Glut-1 and CAIX with the binding of the bioreductive drug hypoxia marker pimonidazole. Pimonidazole was administered to 42 patients with advanced carcinoma of the cervix, 16 hr before biopsy. Sections of single or multiple biopsies were then immunostained for Glut-1 and CAIX, and the area of staining scored by eye, using a "field-by-field" semi-quantitative averaging system. Using I biopsy only, Glut-1 (r = 0.54, p = <0.001) correlated with the level of pimonidazole binding, and Glut-1 and CAIX expression also correlated significantly (r = 0.40, p = <0.009). Thus, our study has shown that HIF-1 regulated genes have potential for future use as predictors of the malignant changes mediated by hypoxia, and warrant further investigation as indicators of response to cancer therapy. © 2002 Wiley-Liss, Inc.
    Original languageEnglish
    Pages (from-to)85-91
    Number of pages6
    JournalInternational Journal of Cancer
    Issue number1
    Publication statusPublished - 10 Mar 2003


    • CAIX
    • Glut-1
    • Hypoxia
    • Pimonidazole


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