Glycoepitopes of staphylococcal wall teichoic acid govern complement-mediated opsonophagocytosis via human serum antibody and mannose-binding lectin

Kenji Kurokawa, Dong Jun Jung, Jang Hyun An, Katharina Fuchs, Yu Jin Jeon, Na Hyang Kim, Xuehua Li, Koichiro Tateishi, Ji Ae Park, Guoqing Xia, Misao Matsushita, Kazue Takahashi, Hee Ju Park, Andreas Peschel, Bok Luel Lee

    Research output: Contribution to journalArticlepeer-review


    Serum antibodies and mannose-binding lectin (MBL) are important host defense factors for host adaptive and innate immunity, respectively. Antibodies and MBL also initiate the classical and lectin complement pathways, respectively, leading to opsonophagocytosis. We have shown previously that Staphylococcus aureus wall teichoic acid (WTA), a cell wall glycopolymer consisting of ribitol phosphate substituted with α- or β-O-N-acetyl-D-glucosamine (GlcNAc) and D-alanine, is recognized by MBL and serum anti-WTA IgG. However, the exact antigenic determinants to which anti-WTA antibodies or MBL bind have not been determined. To answer this question, several S. aureus mutants, such asα-GlcNAc glycosyltransferase-deficientS. aureus AtarM, β-GlcNAc glycosyltransferase-deficient AtarS, and AtarMS double mutant cells, were prepared from a laboratory and a community-associated methicillin-resistant S. aureus strain. Here, we describe the unexpected finding that β-GlcNAc WTA-deficient AtarS mutant cells (which have intact α-GlcNAc) escape from anti-WTA antibody-mediated opsonophagocytosis, whereas α-GlcNAc WTA-deficient AtarM mutant cells (which have intact β-GlcNAc) are efficiently engulfed by human leukocytes via anti-WTA IgG. Likewise, MBL binding in S. aureus cells was lost in the ΔtarMS double mutant but not in either single mutant When we determined the serum concentrations of the anti-α- or anti-β-GlcNAc-specific WTA IgGs, anti-β-GlcNAc WTA-IgG was dominant in pooled human IgG fractions and in the intact sera of healthy adults and infants. These data demonstrate the importance of the WTA sugar conformation for human innate and adaptive immunity against S. aureus infection. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.
    Original languageEnglish
    Pages (from-to)30956-30968
    Number of pages12
    JournalJournal of Biological Chemistry
    Issue number43
    Publication statusPublished - 25 Oct 2013


    • Cell Wall
    • Complement System
    • Gram-positive Bacteria
    • Host Defense
    • Host-Pathogen Interactions
    • Innate Immunity
    • S. aureus


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