Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution

Julie B. Rannes; Avgousta Ioannou; Simon C. Willies; Gideon Grogan; Carsten Behrens; Sabine L. Flitsch; Nicholas J. Turner

doi:10.1021/ja2018477

Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution

Julie B. Rannes, Avgousta Ioannou, Simon C. Willies, Gideon Grogan, Carsten Behrens, Sabine L. Flitsch, Nicholas J. Turner

Research output: Contribution to journal › Article › peer-review

Abstract

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. © 2011 American Chemical Society.

Original language	English
Pages (from-to)	8436-8439
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	22
DOIs	https://doi.org/10.1021/ja2018477
Publication status	Published - 8 Jun 2011

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title = "Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution",

abstract = "A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. {\textcopyright} 2011 American Chemical Society.",

author = "Rannes, {Julie B.} and Avgousta Ioannou and Willies, {Simon C.} and Gideon Grogan and Carsten Behrens and Flitsch, {Sabine L.} and Turner, {Nicholas J.}",

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AU - Rannes, Julie B.

AU - Ioannou, Avgousta

AU - Willies, Simon C.

AU - Grogan, Gideon

AU - Behrens, Carsten

AU - Flitsch, Sabine L.

AU - Turner, Nicholas J.

PY - 2011/6/8

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N2 - A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. © 2011 American Chemical Society.

AB - A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. © 2011 American Chemical Society.

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DO - 10.1021/ja2018477

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SP - 8436

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution

Abstract

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