Abstract
Methods of distinguishing between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by other autofluorescent mols. are described which entail using plane-polarized light to illuminate a sample contg. green fluorescent protein and auto-fluorescent mols.; detecting the intensity of fluorescent light that is emitted with a first polarization from the sample; detecting the intensity of fluorescent light that is emitted with a second polarization from the sample; subtracting a first of the detected intensities from a second of the detected intensities to obtain a difference signal; and using the difference signal to distinguish between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by other fluorescent mols. in the sample. The green fluorescent protein may be expressed in response to DNA damage. [on SciFinder (R)]
| Original language | English |
|---|---|
| Patent number | 2000-514994 6509161 |
| Publication status | Published - 2003 |
Keywords
- Fluorescence (autofluorescence
- distinguishing green fluorescent protein fluorescence from endogenous autofluorescence)
- DNA damage Role: ANT (Analyte), ANST (Analytical study) (detecting and distinguishing fluorescence from green fluorescent protein expressed in response to)
- Fluorometry
- Polarized fluorescence (distinguishing green fluorescent protein fluorescence from endogenous autofluorescence)
- Reporter gene Role: ARU (Analytical role, unclassified), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (for DNA damage
- green fluorescent protein as)
- Proteins Role: ARG (Analytical reagent use), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study), USES (Uses) (green fluorescent
- discriminating between fluorescence from green fluorescent protein from fluorescence from other sources)
- green fluorescent protein fluorescence discrimination endogenous autofluorescence