GSM1574200: 6A

Kim Linton, Christopher Howarth, Mark Wappett, Gillian Newton, Cynthia Lachel, Javeed Iqbal, Stuart Pepper, Richard Byers, Wing J Chan, John Radford

Research output: Non-textual formData set/Database

Abstract

Raw signal intensity data were extracted using Expression Console (Affymetrix). Scatter plots and Pearsons correlation coefficients were used to assess data reproducibility and the strength of association between FT and FFPE-processed tissues. At probe set level, present and absent calls in the first 20 paired FT/FFPE arrays (8 ABC and 12 GCB tumors) were used to calculate the sensitivity and specificity of gene detection in FFPE relative to FT. Probe sets detected exclusively in either FT or FFPE were characterized by relative signal intensity and examined for bias in microarray probe set position. A t-test was used to calculate the proportion of exclusive FT probe sets differentially regulated in ABC and GCB tumors.
Original languageEnglish
PublisherGene Expression Omnibus - GEO
Publication statusPublished - 2014

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