Heat shock proteins and inflammatory acne vulgaris: Molecular cloning, overexpression and purification of a Propionibacterium acnes GroEL and DnaK homologue

Mark D. Farrar; Eileen Ingham; Keith T. Holland

doi:10.1111/j.1574-6968.2000.tb09337.x

Heat shock proteins and inflammatory acne vulgaris: Molecular cloning, overexpression and purification of a Propionibacterium acnes GroEL and DnaK homologue

Mark D. Farrar, Eileen Ingham, Keith T. Holland

Research output: Contribution to journal › Article › peer-review

Abstract

Propionibacterium acnes is associated with inflammatory acne. The genes encoding two putative mediators of inflammation, the heat shock proteins GroEL and DnaK, were cloned from this organism and sequenced. groEL and dnaK encode proteins of 56.8 and 66.4 kDa, respectively, which show a high degree of homology (>75% similarity) to the GroEL and DnaK proteins of mycobacteria and streptomycetes. The promoter regions of both genes contain inverted repeat sequences believed to be involved in the transcriptional regulation of heat shock genes. Recombinant P. acnes GroEL and DnaK were overexpressed in Escherichia coli with C-terminal histidine tags. The recombinant proteins were purified from E. coli by metal affinity chromatography. These proteins will now be used in immunological investigations to determine their role in inflammatory acne. Copyright (C) 2000 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	183-186
Number of pages	3
Journal	FEMS microbiology letters
Volume	191
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2000.tb09337.x
Publication status	Published - 15 Oct 2000

Keywords

Acne vulgaris
DnaK
GroEL
Propionibacterium acnes

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10.1111/j.1574-6968.2000.tb09337.x

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title = "Heat shock proteins and inflammatory acne vulgaris: Molecular cloning, overexpression and purification of a Propionibacterium acnes GroEL and DnaK homologue",

abstract = "Propionibacterium acnes is associated with inflammatory acne. The genes encoding two putative mediators of inflammation, the heat shock proteins GroEL and DnaK, were cloned from this organism and sequenced. groEL and dnaK encode proteins of 56.8 and 66.4 kDa, respectively, which show a high degree of homology (>75% similarity) to the GroEL and DnaK proteins of mycobacteria and streptomycetes. The promoter regions of both genes contain inverted repeat sequences believed to be involved in the transcriptional regulation of heat shock genes. Recombinant P. acnes GroEL and DnaK were overexpressed in Escherichia coli with C-terminal histidine tags. The recombinant proteins were purified from E. coli by metal affinity chromatography. These proteins will now be used in immunological investigations to determine their role in inflammatory acne. Copyright (C) 2000 Federation of European Microbiological Societies.",

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doi = "10.1111/j.1574-6968.2000.tb09337.x",

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T1 - Heat shock proteins and inflammatory acne vulgaris: Molecular cloning, overexpression and purification of a Propionibacterium acnes GroEL and DnaK homologue

AU - Farrar, Mark D.

AU - Ingham, Eileen

AU - Holland, Keith T.

PY - 2000/10/15

Y1 - 2000/10/15

N2 - Propionibacterium acnes is associated with inflammatory acne. The genes encoding two putative mediators of inflammation, the heat shock proteins GroEL and DnaK, were cloned from this organism and sequenced. groEL and dnaK encode proteins of 56.8 and 66.4 kDa, respectively, which show a high degree of homology (>75% similarity) to the GroEL and DnaK proteins of mycobacteria and streptomycetes. The promoter regions of both genes contain inverted repeat sequences believed to be involved in the transcriptional regulation of heat shock genes. Recombinant P. acnes GroEL and DnaK were overexpressed in Escherichia coli with C-terminal histidine tags. The recombinant proteins were purified from E. coli by metal affinity chromatography. These proteins will now be used in immunological investigations to determine their role in inflammatory acne. Copyright (C) 2000 Federation of European Microbiological Societies.

AB - Propionibacterium acnes is associated with inflammatory acne. The genes encoding two putative mediators of inflammation, the heat shock proteins GroEL and DnaK, were cloned from this organism and sequenced. groEL and dnaK encode proteins of 56.8 and 66.4 kDa, respectively, which show a high degree of homology (>75% similarity) to the GroEL and DnaK proteins of mycobacteria and streptomycetes. The promoter regions of both genes contain inverted repeat sequences believed to be involved in the transcriptional regulation of heat shock genes. Recombinant P. acnes GroEL and DnaK were overexpressed in Escherichia coli with C-terminal histidine tags. The recombinant proteins were purified from E. coli by metal affinity chromatography. These proteins will now be used in immunological investigations to determine their role in inflammatory acne. Copyright (C) 2000 Federation of European Microbiological Societies.

KW - Acne vulgaris

KW - DnaK

KW - GroEL

KW - Propionibacterium acnes

U2 - 10.1111/j.1574-6968.2000.tb09337.x

DO - 10.1111/j.1574-6968.2000.tb09337.x

M3 - Article

C2 - 11024261

SN - 1574-6968

VL - 191

SP - 183

EP - 186

JO - FEMS microbiology letters

JF - FEMS microbiology letters

IS - 2

ER -

Heat shock proteins and inflammatory acne vulgaris: Molecular cloning, overexpression and purification of a Propionibacterium acnes GroEL and DnaK homologue

Abstract

Keywords

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