Heterogeneous distribution of DNA alkylation products in rat liver chromatin after in vivo administration of N,N-DI[14C]methylnitrosamine

Helen K. Cooper; G. P. Margison; P. J. O'Connor; Ruth F. Itzhaki

Heterogeneous distribution of DNA alkylation products in rat liver chromatin after in vivo administration of N,N-DI[14C]methylnitrosamine

Helen K. Cooper
, G. P. Margison
, P. J. O'Connor
, Ruth F. Itzhaki

Research output: Contribution to journal › Article › peer-review

Abstract

Poly-l-lysine (PL) binds to about 50% of chromatin DNA, rendering it resistant to degradation by DNAase I. Separation of the unbound DNA as acid-soluble nucleotides allows the fractionation of chromatin DNA into two zones. After in vivo administration of N,N-di[14C]methylnilrosamine, the amount of alkylation in DNA was found to be lower in the polylysine-binding regions. Some possible reasons for this heterogeneous distribution are discussed. © 1975.

Original language	English
Pages (from-to)	483-492
Number of pages	9
Journal	Chemico-Biological Interactions
Volume	11
Issue number	6
Publication status	Published - Dec 1975

Cite this

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title = "Heterogeneous distribution of DNA alkylation products in rat liver chromatin after in vivo administration of N,N-DI[14C]methylnitrosamine",

abstract = "Poly-l-lysine (PL) binds to about 50\% of chromatin DNA, rendering it resistant to degradation by DNAase I. Separation of the unbound DNA as acid-soluble nucleotides allows the fractionation of chromatin DNA into two zones. After in vivo administration of N,N-di[14C]methylnilrosamine, the amount of alkylation in DNA was found to be lower in the polylysine-binding regions. Some possible reasons for this heterogeneous distribution are discussed. {\textcopyright} 1975.",

author = "Cooper, \{Helen K.\} and Margison, \{G. P.\} and O'Connor, \{P. J.\} and Itzhaki, \{Ruth F.\}",

year = "1975",

month = dec,

language = "English",

volume = "11",

pages = "483--492",

journal = "Chemico-Biological Interactions",

issn = "0009-2797",

publisher = "Elsevier BV",

number = "6",

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TY - JOUR

T1 - Heterogeneous distribution of DNA alkylation products in rat liver chromatin after in vivo administration of N,N-DI[14C]methylnitrosamine

AU - Cooper, Helen K.

AU - Margison, G. P.

AU - O'Connor, P. J.

AU - Itzhaki, Ruth F.

PY - 1975/12

Y1 - 1975/12

N2 - Poly-l-lysine (PL) binds to about 50% of chromatin DNA, rendering it resistant to degradation by DNAase I. Separation of the unbound DNA as acid-soluble nucleotides allows the fractionation of chromatin DNA into two zones. After in vivo administration of N,N-di[14C]methylnilrosamine, the amount of alkylation in DNA was found to be lower in the polylysine-binding regions. Some possible reasons for this heterogeneous distribution are discussed. © 1975.

AB - Poly-l-lysine (PL) binds to about 50% of chromatin DNA, rendering it resistant to degradation by DNAase I. Separation of the unbound DNA as acid-soluble nucleotides allows the fractionation of chromatin DNA into two zones. After in vivo administration of N,N-di[14C]methylnilrosamine, the amount of alkylation in DNA was found to be lower in the polylysine-binding regions. Some possible reasons for this heterogeneous distribution are discussed. © 1975.

M3 - Article

C2 - 1201613

SN - 0009-2797

VL - 11

SP - 483

EP - 492

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

IS - 6

ER -

Heterogeneous distribution of DNA alkylation products in rat liver chromatin after in vivo administration of N,N-DI[14C]methylnitrosamine

Abstract

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