HGF/c-Met signalling promotes Notch3 activation and human vascular smooth muscle cell osteogenic differentiation in vitro

Yiwen Liu; Tao Wang; Jianyun Yan; Naomi Jiagbogu; Daniëlle A M Heideman; Ann E. Canfield; M. Yvonne Alexander

doi:10.1016/j.atherosclerosis.2011.08.033

HGF/c-Met signalling promotes Notch3 activation and human vascular smooth muscle cell osteogenic differentiation in vitro

Yiwen Liu, Tao Wang, Jianyun Yan, Naomi Jiagbogu, Daniëlle A M Heideman, Ann E. Canfield, M. Yvonne Alexander

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: Vascular calcification is a major clinical problem and elucidating the underlying mechanism is important to improve the prognosis of patients with cardiovascular disease. We aimed to elucidate the role and mechanism of action of Hepatocyte Growth Factor (HGF)/c-Met signalling in vascular calcification and establish whether blocking this pathway could prevent mineralisation of vascular smooth muscle cells (VSMCs) in vitro. Methods and results: We demonstrate increased HGF secretion and c-Met up-regulation and phosphorylation during VSMC osteogenic differentiation. Adenoviral-mediated over-expression of HGF (AdHGF) in VSMCs accelerated mineralisation, shown by alizarin red staining, and significantly increased 45Calcium incorporation (1.96±0.54-fold [P

Original language	English
Pages (from-to)	440-447
Number of pages	7
Journal	Atherosclerosis
Volume	219
Issue number	2
DOIs	https://doi.org/10.1016/j.atherosclerosis.2011.08.033
Publication status	Published - Dec 2011

Keywords

C-Met
Calcification
Hepatocyte growth factor
NK4
Notch signalling
Vascular smooth muscle cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.atherosclerosis.2011.08.033

Cite this

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title = "HGF/c-Met signalling promotes Notch3 activation and human vascular smooth muscle cell osteogenic differentiation in vitro",

abstract = "Objectives: Vascular calcification is a major clinical problem and elucidating the underlying mechanism is important to improve the prognosis of patients with cardiovascular disease. We aimed to elucidate the role and mechanism of action of Hepatocyte Growth Factor (HGF)/c-Met signalling in vascular calcification and establish whether blocking this pathway could prevent mineralisation of vascular smooth muscle cells (VSMCs) in vitro. Methods and results: We demonstrate increased HGF secretion and c-Met up-regulation and phosphorylation during VSMC osteogenic differentiation. Adenoviral-mediated over-expression of HGF (AdHGF) in VSMCs accelerated mineralisation, shown by alizarin red staining, and significantly increased 45Calcium incorporation (1.96±0.54-fold [P",

keywords = "C-Met, Calcification, Hepatocyte growth factor, NK4, Notch signalling, Vascular smooth muscle cells",

author = "Yiwen Liu and Tao Wang and Jianyun Yan and Naomi Jiagbogu and Heideman, {Dani{\"e}lle A M} and Canfield, {Ann E.} and Alexander, {M. Yvonne}",

year = "2011",

month = dec,

doi = "10.1016/j.atherosclerosis.2011.08.033",

language = "English",

volume = "219",

pages = "440--447",

journal = "Atherosclerosis",

issn = "0021-9150",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - HGF/c-Met signalling promotes Notch3 activation and human vascular smooth muscle cell osteogenic differentiation in vitro

AU - Liu, Yiwen

AU - Wang, Tao

AU - Yan, Jianyun

AU - Jiagbogu, Naomi

AU - Heideman, Daniëlle A M

AU - Canfield, Ann E.

AU - Alexander, M. Yvonne

PY - 2011/12

Y1 - 2011/12

N2 - Objectives: Vascular calcification is a major clinical problem and elucidating the underlying mechanism is important to improve the prognosis of patients with cardiovascular disease. We aimed to elucidate the role and mechanism of action of Hepatocyte Growth Factor (HGF)/c-Met signalling in vascular calcification and establish whether blocking this pathway could prevent mineralisation of vascular smooth muscle cells (VSMCs) in vitro. Methods and results: We demonstrate increased HGF secretion and c-Met up-regulation and phosphorylation during VSMC osteogenic differentiation. Adenoviral-mediated over-expression of HGF (AdHGF) in VSMCs accelerated mineralisation, shown by alizarin red staining, and significantly increased 45Calcium incorporation (1.96±0.54-fold [P

AB - Objectives: Vascular calcification is a major clinical problem and elucidating the underlying mechanism is important to improve the prognosis of patients with cardiovascular disease. We aimed to elucidate the role and mechanism of action of Hepatocyte Growth Factor (HGF)/c-Met signalling in vascular calcification and establish whether blocking this pathway could prevent mineralisation of vascular smooth muscle cells (VSMCs) in vitro. Methods and results: We demonstrate increased HGF secretion and c-Met up-regulation and phosphorylation during VSMC osteogenic differentiation. Adenoviral-mediated over-expression of HGF (AdHGF) in VSMCs accelerated mineralisation, shown by alizarin red staining, and significantly increased 45Calcium incorporation (1.96±0.54-fold [P

KW - C-Met

KW - Calcification

KW - Hepatocyte growth factor

KW - NK4

KW - Notch signalling

KW - Vascular smooth muscle cells

U2 - 10.1016/j.atherosclerosis.2011.08.033

DO - 10.1016/j.atherosclerosis.2011.08.033

M3 - Article

C2 - 21920521

SN - 0021-9150

VL - 219

SP - 440

EP - 447

JO - Atherosclerosis

JF - Atherosclerosis

IS - 2

ER -

HGF/c-Met signalling promotes Notch3 activation and human vascular smooth muscle cell osteogenic differentiation in vitro

Abstract

Keywords

UN SDGs

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