High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex

Sara Gago; Óscar Zaragoza; Isabel Cuesta; Juan L Rodríguez-Tudela; Manuel Cuenca-Estrella; María J Buitrago

doi:10.1128/JCM.01091-11

High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex

Sara Gago, Óscar Zaragoza, Isabel Cuesta, Juan L Rodríguez-Tudela, Manuel Cuenca-Estrella, María J Buitrago

Division of Immunology, Immunity to Infection and Respiratory Medicine

Instituto de Salud Carlos III

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

Original language	English
Pages (from-to)	3663-6
Number of pages	4
Journal	Journal of clinical microbiology
Volume	49
Issue number	10
DOIs	https://doi.org/10.1128/JCM.01091-11
Publication status	Published - Oct 2011

Keywords

Clinical Laboratory Techniques/methods
Cryptococcosis/diagnosis
Cryptococcus gattii/classification
Cryptococcus neoformans/classification
DNA, Fungal/chemistry
Humans
Mycology/methods
Sensitivity and Specificity
Temperature
Time Factors
Transition Temperature

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10.1128/JCM.01091-11

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title = "High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex",

abstract = "We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.",

keywords = "Clinical Laboratory Techniques/methods, Cryptococcosis/diagnosis, Cryptococcus gattii/classification, Cryptococcus neoformans/classification, DNA, Fungal/chemistry, Humans, Mycology/methods, Sensitivity and Specificity, Temperature, Time Factors, Transition Temperature",

author = "Sara Gago and {\'O}scar Zaragoza and Isabel Cuesta and Rodr{\'i}guez-Tudela, {Juan L} and Manuel Cuenca-Estrella and Buitrago, {Mar{\'i}a J}",

year = "2011",

month = oct,

doi = "10.1128/JCM.01091-11",

language = "English",

volume = "49",

pages = "3663--6",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex

AU - Gago, Sara

AU - Zaragoza, Óscar

AU - Cuesta, Isabel

AU - Rodríguez-Tudela, Juan L

AU - Cuenca-Estrella, Manuel

AU - Buitrago, María J

PY - 2011/10

Y1 - 2011/10

N2 - We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

AB - We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

KW - Clinical Laboratory Techniques/methods

KW - Cryptococcosis/diagnosis

KW - Cryptococcus gattii/classification

KW - Cryptococcus neoformans/classification

KW - DNA, Fungal/chemistry

KW - Humans

KW - Mycology/methods

KW - Sensitivity and Specificity

KW - Temperature

KW - Time Factors

KW - Transition Temperature

U2 - 10.1128/JCM.01091-11

DO - 10.1128/JCM.01091-11

M3 - Article

C2 - 21832024

SN - 0095-1137

VL - 49

SP - 3663

EP - 3666

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 10

ER -

High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex

Abstract

Keywords

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