High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing - cryoprinting

Rui Dou; Rachel Saunders; L Mohamet; Christopher M Ward; Brian Derby

doi:10.1039/C5LC00674K

High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing - cryoprinting

Rui Dou, Rachel Saunders, L Mohamet, Christopher M Ward, Brian Derby

Division of Dentistry (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotection agent (CPA) at rates in the region 100 Hz-20 kHz, from individual droplets of 380 pl. After printing and a freeze/thaw cycle (with a minimum 24 hours hold period at liquid N2 temperature), 3T3 cells showed an average viability of >90% with CPA concentration

Original language	English
Pages (from-to)	3503-3513
Number of pages	10
Journal	Lab on a Chip
Volume	15
Issue number	17
DOIs	https://doi.org/10.1039/C5LC00674K
Publication status	Published - 7 Sept 2015

Keywords

inkjet
cell printing
cryopreservation
bioprinting

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10.1039/C5LC00674K

Cite this

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title = "High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing - cryoprinting",

abstract = "We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotection agent (CPA) at rates in the region 100 Hz-20 kHz, from individual droplets of 380 pl. After printing and a freeze/thaw cycle (with a minimum 24 hours hold period at liquid N2 temperature), 3T3 cells showed an average viability of >90% with CPA concentration",

keywords = "inkjet, cell printing, cryopreservation, bioprinting",

author = "Rui Dou and Rachel Saunders and L Mohamet and Ward, {Christopher M} and Brian Derby",

note = "We would like to acknowledge the support of the EPSRC through project grants EP/L012022/1, EP/C01328X/1, EP/ H046070/1 and BBSRC follow-on-fund BB/K020277/1. RES would like to acknowledge support from the EPSRC for a Fel- lowship through grant EP/G051259/1.",

year = "2015",

month = sep,

day = "7",

doi = "10.1039/C5LC00674K",

language = "English",

volume = "15",

pages = "3503--3513",

journal = "Lab on a Chip",

issn = "1473-0189",

publisher = "Royal Society of Chemistry",

number = "17",

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T1 - High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing - cryoprinting

AU - Dou, Rui

AU - Saunders, Rachel

AU - Mohamet, L

AU - Ward, Christopher M

AU - Derby, Brian

N1 - We would like to acknowledge the support of the EPSRC through project grants EP/L012022/1, EP/C01328X/1, EP/ H046070/1 and BBSRC follow-on-fund BB/K020277/1. RES would like to acknowledge support from the EPSRC for a Fel- lowship through grant EP/G051259/1.

PY - 2015/9/7

Y1 - 2015/9/7

N2 - We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotection agent (CPA) at rates in the region 100 Hz-20 kHz, from individual droplets of 380 pl. After printing and a freeze/thaw cycle (with a minimum 24 hours hold period at liquid N2 temperature), 3T3 cells showed an average viability of >90% with CPA concentration

AB - We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotection agent (CPA) at rates in the region 100 Hz-20 kHz, from individual droplets of 380 pl. After printing and a freeze/thaw cycle (with a minimum 24 hours hold period at liquid N2 temperature), 3T3 cells showed an average viability of >90% with CPA concentration

KW - inkjet

KW - cell printing

KW - cryopreservation

KW - bioprinting

U2 - 10.1039/C5LC00674K

DO - 10.1039/C5LC00674K

M3 - Article

SN - 1473-0189

VL - 15

SP - 3503

EP - 3513

JO - Lab on a Chip

JF - Lab on a Chip

IS - 17

ER -

High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing - cryoprinting

Abstract

Keywords

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