Highly branched stimuli responsive poly[(N-isopropyl acrylamide)-co-(1,2- propandiol-3-methacrylate)]s with protein binding functionality

Steven Carter, Stephen Rimmer, Alice Sturdy, Michelle Webb

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive "smart" polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA.
    Original languageEnglish
    Pages (from-to)373-378
    Number of pages5
    JournalMacromolecular Bioscience
    Volume5
    Issue number5
    DOIs
    Publication statusPublished - 23 May 2005

    Keywords

    • Affinity precipitation
    • Copolymerization
    • His-tagged protein
    • Reversible addition-fragmentation chain transfer (RAFT)
    • Water-soluble polymers

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