Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive "smart" polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA.
- Affinity precipitation
- His-tagged protein
- Reversible addition-fragmentation chain transfer (RAFT)
- Water-soluble polymers