Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen

Michael R. Chapman, Sebastian C. Cosgrove, Nicholas J. Turner, Nikil Kapur, A. John Blacker

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    Abstract

    We report a simple, mild, and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H2O2 is used to produce a soluble source of O2 directly in reaction media, thereby enabling the concentration of aqueous O2 to be increased beyond equilibrium solubility under safe and practical conditions. To best exploit this method, a novel flow reactor was developed to maximize productivity (g product L−1 h−1). This scalable benchtop method provides a distinct advantage over conventional bio-oxidation in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space-time yields for bio-oxidation.

    Original languageEnglish
    Pages (from-to)10535-10539
    Number of pages5
    JournalAngewandte Chemie - International Edition
    Volume57
    Issue number33
    Early online date9 May 2018
    DOIs
    Publication statusPublished - 13 Aug 2018

    Keywords

    • biocatalysis
    • continuous flow
    • flow reactors
    • oxidation
    • space-time yields

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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