Histochemical analysis of rat testicular glycoconjugates. 1. Subsets of N-linked saccharides in seminiferous tubules

Carolyn Jones, C.A. Morrison, R.W. Stoddart

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The distribution of N-linked glycans in rat testis has been probed using a panel of lectins derived from Galanthus nivalis (snowdrop, GNA), Canavalia ensiformis (jack bean, Con A), Lens culinaris (lentil, LCA), Pisum sativum (garden pea, PSA) and Phaseolus vulgaris, erythro- and leucoagglutinins (kidney bean, ePHA and IPHA). Several classes of N-linked glycan were identified in the spermatogenic series, and during differentiation into spermatozoa they altered in both their pattern of distribution and relative abundance. A population of tetra-antennary, non-bisected, complex glycans, detected by IPHA, was lost during the transition from spermatogonia to spermatocytes, while high-mannose structures were acquired; these were most abundant in spermatocytes, as were bi- and tri-antennary complex, non-bisected glycans, the latter becoming increasingly abundant on acrosomes and spermatozoa. Their bisected counterparts were more generally expressed throughout spermatogenic cells, although marked localization onto acrosomes and nuclear caps was again seen. Transition from spermatocytes to spermatids involved mainly changes of the acrosomal granule and nuclear cap, which were carried through to the final stages of differentiation. Sertoli cell surfaces and cytoplasmic granules showed a high level of N-glycan expression. © 1992 Chapman & Hall.
    Original languageUndefined
    Pages (from-to)319-326
    Number of pages8
    JournalThe Histochemical Journal
    Volume24
    Issue number6
    DOIs
    Publication statusPublished - 1992

    Keywords

    • asparagine linked oligosaccharide
    • glycan
    • glycoconjugate
    • lectin
    • mannose, acrosome
    • animal tissue
    • article
    • cell differentiation
    • cell nucleus
    • cell surface
    • histochemistry
    • male
    • nonhuman
    • priority journal
    • rat
    • seminiferous tubule
    • sertoli cell
    • spermatid
    • spermatocyte
    • spermatogenesis
    • spermatozoon
    • testis
    • tissue distribution, Animal
    • Galanthus
    • Glycoconjugates
    • Histocytochemistry
    • Lectins
    • Male
    • Polysaccharides
    • Rats
    • Seminiferous Tubules
    • Spermatogenesis
    • Spermatozoa
    • Testis, Animalia

    Cite this