Projects per year
trinuclear magnesium cluster and pyrophosphate. Computational modelling, experiment, and bioinformatic analysis identified residues important in steering the reaction chemistry. We show that S206 is crucial in 10-epi-cubebol synthesis by enlisting the nearby F211 to shape the active site contour and prevent the formation of early escape cadalane products. We also show that N327 and F104 control the distribution between several early-stage cations andwhether the final product is derived from the germacrane, cadalane, or cubebane hydrocarbon scaffold. Using these insights, we re-engineered ScCubS so that its main product
was germacradien-4-ol, which derives from the germacrane, rather than the cubebane, scaffold. Our work emphasises that mechanistic understanding of cation stabilisation in TSs can be used to guide catalytic outcomes.
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology
FingerprintDive into the research topics of 'How a 10-epi-cubebol Synthase Avoids Premature Reaction Quenching to Form a Tricyclic Product at High Purity'. Together they form a unique fingerprint.
- 3 Finished
Scrutton, N., Azapagic, A., Balmer, A., Barran, P., Breitling, R., Delneri, D., Dixon, N., Faulon, J., Flitsch, S., Goble, C., Goodacre, R., Hay, S., Kell, D., Leys, D., Lloyd, J., Lockyer, N., Martin, P., Micklefield, J., Munro, A., Pedrosa Mendes, P., Randles, S., Salehi Yazdi, F., Shapira, P., Takano, E., Turner, N. & Winterburn, J.
14/11/14 → 13/05/20
1/07/14 → 30/06/17