HSV-tk gene therapy for human renal cell carcinoma in nude mice

Kalevi Juhani Pulkkanen; Jyrki J. Parkkinen; Johanna M. Laukkanen; Mikko I. Kettunen; Kristiina Tyynela; Risto A. Kauppinen; Martti Y. Ala-Opas; Seppo Yla-Herttuala

doi:10.1038/sj.cgt.7700342

HSV-tk gene therapy for human renal cell carcinoma in nude mice

Kalevi Juhani Pulkkanen, Jyrki J. Parkkinen, Johanna M. Laukkanen, Mikko I. Kettunen, Kristiina Tyynela, Risto A. Kauppinen, Martti Y. Ala-Opas, Seppo Yla-Herttuala

Research output: Contribution to journal › Article › peer-review

Abstract

We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus- mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22 ± 14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P

Original language	English
Pages (from-to)	529-536
Number of pages	7
Journal	Cancer Gene Therapy
Volume	8
Issue number	7
DOIs	https://doi.org/10.1038/sj.cgt.7700342
Publication status	Published - 2001

Keywords

Gene therapy
HSV-tk
Kidney carcinoma

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.cgt.7700342

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title = "HSV-tk gene therapy for human renal cell carcinoma in nude mice",

abstract = "We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus- mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22 ± 14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P",

keywords = "Gene therapy, HSV-tk, Kidney carcinoma",

author = "Pulkkanen, {Kalevi Juhani} and Parkkinen, {Jyrki J.} and Laukkanen, {Johanna M.} and Kettunen, {Mikko I.} and Kristiina Tyynela and Kauppinen, {Risto A.} and Ala-Opas, {Martti Y.} and Seppo Yla-Herttuala",

year = "2001",

doi = "10.1038/sj.cgt.7700342",

language = "English",

volume = "8",

pages = "529--536",

journal = "Cancer Gene Therapy",

issn = "1476-5500",

publisher = "Springer Nature",

number = "7",

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TY - JOUR

T1 - HSV-tk gene therapy for human renal cell carcinoma in nude mice

AU - Pulkkanen, Kalevi Juhani

AU - Parkkinen, Jyrki J.

AU - Laukkanen, Johanna M.

AU - Kettunen, Mikko I.

AU - Tyynela, Kristiina

AU - Kauppinen, Risto A.

AU - Ala-Opas, Martti Y.

AU - Yla-Herttuala, Seppo

PY - 2001

Y1 - 2001

N2 - We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus- mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22 ± 14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P

AB - We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus- mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22 ± 14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P

KW - Gene therapy

KW - HSV-tk

KW - Kidney carcinoma

U2 - 10.1038/sj.cgt.7700342

DO - 10.1038/sj.cgt.7700342

M3 - Article

C2 - 11498775

SN - 1476-5500

VL - 8

SP - 529

EP - 536

JO - Cancer Gene Therapy

JF - Cancer Gene Therapy

IS - 7

ER -

HSV-tk gene therapy for human renal cell carcinoma in nude mice

Abstract

Keywords

UN SDGs

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