Human colostrum: Identification of minor proteins in the aqueous phase by proteomics

David J. Palmer; Van C. Kelly; Anne Marie Smit; Sulee Kuy; Catriona G. Knight; Garth Cooper

doi:10.1002/pmic.200500558

Human colostrum: Identification of minor proteins in the aqueous phase by proteomics

David J. Palmer, Van C. Kelly, Anne Marie Smit, Sulee Kuy, Catriona G. Knight, Garth Cooper

Division of Cardiovascular Sciences (L5)

University of Auckland

Research output: Contribution to journal › Article › peer-review

Abstract

Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-D LC and gel-based proteomic methods. One hundred and fifty-one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48 h of lactation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

Original language	English
Pages (from-to)	2208-2216
Number of pages	8
Journal	Proteomics
Volume	6
Issue number	7
DOIs	https://doi.org/10.1002/pmic.200500558
Publication status	Published - Apr 2006

Keywords

Colostrum
Human
Proteome

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title = "Human colostrum: Identification of minor proteins in the aqueous phase by proteomics",

abstract = "Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-D LC and gel-based proteomic methods. One hundred and fifty-one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48 h of lactation. {\textcopyright} 2006 Wiley-VCH Verlag GmbH & Co. KGaA.",

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author = "Palmer, {David J.} and Kelly, {Van C.} and Smit, {Anne Marie} and Sulee Kuy and Knight, {Catriona G.} and Garth Cooper",

year = "2006",

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AU - Palmer, David J.

AU - Kelly, Van C.

AU - Smit, Anne Marie

AU - Kuy, Sulee

AU - Knight, Catriona G.

AU - Cooper, Garth

PY - 2006/4

Y1 - 2006/4

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AB - Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-D LC and gel-based proteomic methods. One hundred and fifty-one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48 h of lactation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

KW - Colostrum

KW - Human

KW - Proteome

U2 - 10.1002/pmic.200500558

DO - 10.1002/pmic.200500558

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SN - 1615-9861

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SP - 2208

EP - 2216

JO - Proteomics

JF - Proteomics

IS - 7

ER -

Human colostrum: Identification of minor proteins in the aqueous phase by proteomics

Abstract

Keywords

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