Human feeder cell line for derivation and culture of hESc/hiPSc

Tristan R McKay; Maria V Camarasa; Banu Iskender; Jinpei Ye; Nicola Bates; Duncan Miller; Jayne C Fitzsimmons; Daniel Foxler; Maureen Mee; Tyson V Sharp; John Aplin; Daniel R Brison; Susan J Kimber

doi:10.1016/j.scr.2011.04.005

Human feeder cell line for derivation and culture of hESc/hiPSc

Tristan R McKay
, Maria V Camarasa
, Banu Iskender
, Jinpei Ye
, Nicola Bates
, Duncan Miller
, Jayne C Fitzsimmons
, Daniel Foxler
, Maureen Mee
, Tyson V Sharp
, John Aplin
, Daniel R Brison
, Susan J Kimber

Research output: Contribution to journal › Article › peer-review

Abstract

We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

Original language	English
Pages (from-to)	154-162
Number of pages	9
Journal	Stem Cell Research
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/j.scr.2011.04.005 https://doi.org/10.1016/j.scr.2011.04.005
Publication status	Published - Sept 2011

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title = "Human feeder cell line for derivation and culture of hESc/hiPSc",

abstract = "We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.",

author = "McKay, \{Tristan R\} and Camarasa, \{Maria V\} and Banu Iskender and Jinpei Ye and Nicola Bates and Duncan Miller and Fitzsimmons, \{Jayne C\} and Daniel Foxler and Maureen Mee and Sharp, \{Tyson V\} and John Aplin and Brison, \{Daniel R\} and Kimber, \{Susan J\}",

year = "2011",

month = sep,

doi = "10.1016/j.scr.2011.04.005",

language = "English",

volume = "7",

pages = "154--162",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Human feeder cell line for derivation and culture of hESc/hiPSc

AU - McKay, Tristan R

AU - Camarasa, Maria V

AU - Iskender, Banu

AU - Ye, Jinpei

AU - Bates, Nicola

AU - Miller, Duncan

AU - Fitzsimmons, Jayne C

AU - Foxler, Daniel

AU - Mee, Maureen

AU - Sharp, Tyson V

AU - Aplin, John

AU - Brison, Daniel R

AU - Kimber, Susan J

PY - 2011/9

Y1 - 2011/9

N2 - We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

AB - We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

U2 - 10.1016/j.scr.2011.04.005

DO - 10.1016/j.scr.2011.04.005

M3 - Article

C2 - 21763623

SN - 1873-5061

VL - 7

SP - 154

EP - 162

JO - Stem Cell Research

JF - Stem Cell Research

IS - 2

ER -

Human feeder cell line for derivation and culture of hESc/hiPSc

Abstract

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