Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation

Aoife Kelly, Sezin Gunaltay, Craig P. Mcentee, Elinor E. Shuttleworth, Catherine Smedley, Stephanie A. Houston, Thomas M. Fenton, Scott Levison, Elizabeth R. Mann, Mark A. Travis

Research output: Contribution to journalArticlepeer-review


Monocytes are crucial immune cells involved in regulation of inflammation either directly or via differentiation into macrophages in tissues. However, many aspects of how their function is controlled in health and disease are not understood. Here we show that human blood monocytes activate high levels of the cytokine TGFβ, a pathway that is not evident in mouse monocytes. Human CD14+, but not CD16+, monocytes activate TGFβ via expression of the integrin αvβ8 and matrix metalloproteinase 14, which dampens their production of TNFα in response to LPS. Additionally, when monocytes differentiate into macrophages, integrin expression and TGFβ-activating ability are maintained in anti-inflammatory macrophages but down-regulated in pro-inflammatory macrophages. In the healthy human intestine, integrin αvβ8 is highly expressed on mature tissue macrophages, with these cells and their integrin expression being significantly reduced in active inflammatory bowel disease. Thus, our data suggest that integrin αvβ8–mediated TGFβ activation plays a key role in regulation of monocyte inflammatory responses and intestinal macrophage homeostasis.
Original languageEnglish
Pages (from-to)2725-2736
Number of pages12
JournalThe Journal of experimental medicine
Issue number11
Early online date24 Oct 2018
Publication statusPublished - 24 Oct 2018

Research Beacons, Institutes and Platforms

  • Lydia Becker Institute


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