Hydrogen peroxide and endothelium-dependent hyperpolarization in the guinea-pig carotid artery

Pascale Gluais; Gillian Edwards; Arthur H. Weston; Paul M. Vanhoutte; Michel Félétou

doi:10.1016/j.ejphar.2005.02.042

Hydrogen peroxide and endothelium-dependent hyperpolarization in the guinea-pig carotid artery

Pascale Gluais, Gillian Edwards, Arthur H. Weston, Paul M. Vanhoutte, Michel Félétou

Research output: Contribution to journal › Article › peer-review

Abstract

This study was designed to determine whether or not endothelium-dependent hyperpolarizations evoked by acetylcholine in the isolated guinea-pig carotid artery involve hydrogen peroxide. Membrane potential was recorded in the vascular smooth muscle cells of that artery. Under control conditions, acetylcholine induced endothelium-dependent hyperpolarization of the vascular smooth muscle cells which was not affected by the presence of catalase, superoxide dismutase or their combination. Neither the superoxide dismutase mimetic, tiron nor the thiol-reducing agent N-acetyl-l-cysteine modified the hyperpolarization evoked by 0.1 μM acetylcholine but each produced a partial and significant inhibition of the hyperpolarization induced by 1 μM acetylcholine. Neither 10 nor 100 μM hydrogen peroxide influenced the resting membrane potential of the smooth muscle cells and the higher concentration did not significantly influence the hyperpolarization elicited by acetylcholine. These data indicate that, in the guinea-pig isolated carotid artery, hydrogen peroxide is unlikely to contribute to the endothelium-dependent hyperpolarization evoked by acetylcholine. © 2005 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	219-224
Number of pages	5
Journal	European journal of pharmacology
Volume	513
Issue number	3
DOIs	https://doi.org/10.1016/j.ejphar.2005.02.042
Publication status	Published - 25 Apr 2005

Keywords

Endothelium
Endothelium-derived hyperpolarizing factor
Hydrogen peroxide
Membrane potential
Smooth muscle

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10.1016/j.ejphar.2005.02.042

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title = "Hydrogen peroxide and endothelium-dependent hyperpolarization in the guinea-pig carotid artery",

abstract = "This study was designed to determine whether or not endothelium-dependent hyperpolarizations evoked by acetylcholine in the isolated guinea-pig carotid artery involve hydrogen peroxide. Membrane potential was recorded in the vascular smooth muscle cells of that artery. Under control conditions, acetylcholine induced endothelium-dependent hyperpolarization of the vascular smooth muscle cells which was not affected by the presence of catalase, superoxide dismutase or their combination. Neither the superoxide dismutase mimetic, tiron nor the thiol-reducing agent N-acetyl-l-cysteine modified the hyperpolarization evoked by 0.1 μM acetylcholine but each produced a partial and significant inhibition of the hyperpolarization induced by 1 μM acetylcholine. Neither 10 nor 100 μM hydrogen peroxide influenced the resting membrane potential of the smooth muscle cells and the higher concentration did not significantly influence the hyperpolarization elicited by acetylcholine. These data indicate that, in the guinea-pig isolated carotid artery, hydrogen peroxide is unlikely to contribute to the endothelium-dependent hyperpolarization evoked by acetylcholine. {\textcopyright} 2005 Elsevier B.V. All rights reserved.",

keywords = "Endothelium, Endothelium-derived hyperpolarizing factor, Hydrogen peroxide, Membrane potential, Smooth muscle",

author = "Pascale Gluais and Gillian Edwards and Weston, {Arthur H.} and Vanhoutte, {Paul M.} and Michel F{\'e}l{\'e}tou",

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AU - Gluais, Pascale

AU - Edwards, Gillian

AU - Weston, Arthur H.

AU - Vanhoutte, Paul M.

AU - Félétou, Michel

PY - 2005/4/25

Y1 - 2005/4/25

N2 - This study was designed to determine whether or not endothelium-dependent hyperpolarizations evoked by acetylcholine in the isolated guinea-pig carotid artery involve hydrogen peroxide. Membrane potential was recorded in the vascular smooth muscle cells of that artery. Under control conditions, acetylcholine induced endothelium-dependent hyperpolarization of the vascular smooth muscle cells which was not affected by the presence of catalase, superoxide dismutase or their combination. Neither the superoxide dismutase mimetic, tiron nor the thiol-reducing agent N-acetyl-l-cysteine modified the hyperpolarization evoked by 0.1 μM acetylcholine but each produced a partial and significant inhibition of the hyperpolarization induced by 1 μM acetylcholine. Neither 10 nor 100 μM hydrogen peroxide influenced the resting membrane potential of the smooth muscle cells and the higher concentration did not significantly influence the hyperpolarization elicited by acetylcholine. These data indicate that, in the guinea-pig isolated carotid artery, hydrogen peroxide is unlikely to contribute to the endothelium-dependent hyperpolarization evoked by acetylcholine. © 2005 Elsevier B.V. All rights reserved.

AB - This study was designed to determine whether or not endothelium-dependent hyperpolarizations evoked by acetylcholine in the isolated guinea-pig carotid artery involve hydrogen peroxide. Membrane potential was recorded in the vascular smooth muscle cells of that artery. Under control conditions, acetylcholine induced endothelium-dependent hyperpolarization of the vascular smooth muscle cells which was not affected by the presence of catalase, superoxide dismutase or their combination. Neither the superoxide dismutase mimetic, tiron nor the thiol-reducing agent N-acetyl-l-cysteine modified the hyperpolarization evoked by 0.1 μM acetylcholine but each produced a partial and significant inhibition of the hyperpolarization induced by 1 μM acetylcholine. Neither 10 nor 100 μM hydrogen peroxide influenced the resting membrane potential of the smooth muscle cells and the higher concentration did not significantly influence the hyperpolarization elicited by acetylcholine. These data indicate that, in the guinea-pig isolated carotid artery, hydrogen peroxide is unlikely to contribute to the endothelium-dependent hyperpolarization evoked by acetylcholine. © 2005 Elsevier B.V. All rights reserved.

KW - Endothelium

KW - Endothelium-derived hyperpolarizing factor

KW - Hydrogen peroxide

KW - Membrane potential

KW - Smooth muscle

U2 - 10.1016/j.ejphar.2005.02.042

DO - 10.1016/j.ejphar.2005.02.042

M3 - Article

C2 - 15862803

SN - 0014-2999

VL - 513

SP - 219

EP - 224

JO - European journal of pharmacology

JF - European journal of pharmacology

IS - 3

ER -

Hydrogen peroxide and endothelium-dependent hyperpolarization in the guinea-pig carotid artery

Abstract

Keywords

UN SDGs

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